Caracterização de membranas de colágeno modificado com extrato de frutos de Genipa americana L. e avaliação histomorfológica do efeito sobre o processo de reparo cicatricial por segunda intenção em ratos

Content Provider	Semantic Scholar
Author	Neto, Rezende Melquiades, José Antonio Rodríguez
Copyright Year	2011
Abstract	Biological membranes produced from biomaterials has been widely studied because of their applications in the pharmaceutical industry. The goal of this study was to characterize collagen membranes modified with methanol extract of fruits of G. americana L, called "genipina", supplemented with ethanol extract of propolis and evaluate its potential healing in rodents. The membranes produced by "casting process" from this polymer (COL, COLGEN and COLGENP) were characterized according to their morphological characteristics. The evaluation of the healing process of wounds was performed in 80 Wistar rats separated into the following groups: CTR wounds without cobertrura; COL wounds covered with collagen membrane; COLGEN wounds covered with collagen membrane crosslinked with genipina; COLGENP wounds covered membrane collagen cross-linked with genipina added in 0.5% extract of propolis. The animals were sacrificed at 3, 7, 14 and 21 days and the wounds removed and examined histologically. We assessed the rate of wound contraction (ICF), the intensity of the inflammatory response (IRI) and the rate of wound collagen (ICG). The data from IRI were analyzed using the Kruskal Wallis test, extending post-hoc Dunn, while the ICF ICG and were analyzed by ANOVA with post-hoc extension of Tukey. The level of significance was set at 5% (p <0.05). It was observed that had draped the maximum stress of rupture and modulus of elasticity over COLGEN and COLGENP. In the trial of healing, it was noted that ICF COLGENP induced higher CTR than 7 days (p = 0.04). There was no significant difference in IRI between the groups (p <0.05), but both COL, COLGEN COLGENP and promoted faster chronicity of the inflammatory process. The ICG COLGENP was significantly higher than in CTR in 7 days (0.04, respectively), but there were no differences in other experimental times. Moreover COLGENP caused early replacement of type II collagen by type I, and better organization of the architectural type I collagen fibers at the end of the experiment. It was concluded that the crosslinking provided the production of plastic membranes most probably due to decreased interactions intracadeias. The collagen membranes, collagen and collagen cross-linked with cross-linked with genipina genipina more propolis proved effective for use as bandages ("dressings") of open wounds. While the results obtained with both membranes were statistically similar, it is possible to infer that the chemical crosslinking of the collagen molecule did not alter the pro-healing properties of the membrane, as well as the addition of propolis. Key-Words: Biological dressing, collagen, wound healing.
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Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article