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Effects of rare earth compounds on growth and apoptosis of leukemic cell lines
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dai, Yucheng Li, Jian Qiang Hu, Aiguo Yuan, Liya |
| Copyright Year | 2007 |
| Abstract | SummaryTo explore a new agent for inhibiting leukemic cells, we investigated the effects of rare earth compounds (lanthanum chloride and cerium chloride) on the growth and apoptosis of HL-60 and NB4 cells. The growth of HL-60 and NB4 cells was tested by 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) colorimetric assay. The apoptosis was measured by light microscopy, flow cytometry, and terminal deoxynucleotidyl transferase—mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) method. The effect of LaCl3 on normal bone marrow hematopoietic progenitor cells was evaluated by colony-forming unit-granulocyte-macrophage (CFU-GM) assay. Under our experimental conditions, MTT assay showed that 48-h treatment with 1, 2, and 3 mM LaCl3 or 48- and 72-h treatments with 1 mM LaCl3 could significantly inhibit the growth of HL-60 cells. Treatment with 2 and 4 mM CeCl3 for 72 h could significantly inhibit the growth of NB4 cells. Apoptosis could be detected on treatment with 2 mM LaCl3 for 24 h in HL-60 cells by light microscopic morphology examination, flow cytometric analysis, and TUNEL method. Apoptosis could be also detected on treatment with 2 mM CeCl3 for 72 h in NB4 cells. Treatment with 1 mM LaCl3 could arrest the transitions from GO/G1 to S phase. The granulocyte-macrophage colony formation of normal bone marrow cells was not significantly inhibited at lower concentrations of LaCl3 (0.5 to 2 mM). Our results indicate that at certain concentrations, the rare earth compounds may inhibit the growth of leukemic cells, induce them to apoptosis, and have no significant inhibitory effects on normal bone marrow hematopoietic progenitor cells (CFU-GM). The mechanism needs to be further investigated. |
| Starting Page | 373 |
| Ending Page | 375 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| DOI | 10.1290/1071-2690(2002)038<0373:EORECO>2.0.CO;2 |
| PubMed reference number | 12534335 |
| Journal | Medline |
| Volume Number | 38 |
| Alternate Webpage(s) | https://page-one.live.cf.public.springer.com/pdf/preview/10.1290/1071-2690(2002)038%3C0373:EORECO%3E2.0.CO;2 |
| Alternate Webpage(s) | https://doi.org/10.1290/1071-2690%282002%29038%3C0373%3AEORECO%3E2.0.CO%3B2 |
| Journal | In Vitro Cellular & Developmental Biology - Animal |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
