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Pontibacter diazotrophicus sp. nov., a Novel Nitrogen-Fixing Bacterium of the Family Cytophagaceae
Content Provider | Semantic Scholar |
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Author | Xu, Linghua Zeng, Xian-Chun Luo, Xuesong Zhou, En-Min Zhou, Lingli Pan, Yunfan Li, Wen-Jun |
Copyright Year | 2014 |
Abstract | Few diazotrophs have been found to belong to the family Cytophagaceae so far. In the present study, a Gram-negative, rod-shaped bacterium that forms red colonies, was isolated from sands of the Takalamakan desert. It was designated H4XT. Phylogenetic and biochemical analysis indicated that the isolate is a new species of the genus Pontibacter. The 16S rRNA gene of H4XT displays 94.2-96.8% sequence similarities to those of other strains in Pontibacter. The major respiratory quinone is menaquinone-7 (MK-7). The DNA G+C content is 46.6 mol%. The major cellular fatty acids are iso-C15∶0, C16∶1ω5c, summed feature 3 (containing C16∶1ω6c and/or C16∶1ω7c) and summed feature 4 (comprising anteiso-C17∶1B and/or iso-C17∶1I). The major polar lipids are phosphatidylethanolamine (PE), one aminophospholipid (APL) and some unknown phospholipids (PLs). It is interesting to see that this bacterium can grow very well in a nitrogen-free medium. PCR amplification suggested that the bacterium possesses at least one type of nitrogenase gene. Acetylene reduction assay showed that H4XT actually possesses nitrogen-fixing activity. Therefore, it can be concluded that H4XT is a new diazotroph. We thus referred it to as Pontibacter diazotrophicus sp. nov. The type strain is H4XT ( = CCTCC AB 2013049T = NRRL B-59974T). |
File Format | PDF HTM / HTML |
DOI | 10.1371/journal.pone.0092294 |
PubMed reference number | 24647674 |
Journal | Medline |
Volume Number | 9 |
Alternate Webpage(s) | http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0092294&type=printable |
Alternate Webpage(s) | https://doi.org/10.1371/journal.pone.0092294 |
Journal | PloS one |
Language | English |
Access Restriction | Open |
Content Type | Text |
Resource Type | Article |