NDLI: A novel screening method for cell wall mutants in Aspergillus niger identifies UDP-galactopyranose mutase as an important protein in fungal cell wall biosynthesis.

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A novel screening method for cell wall mutants in Aspergillus niger identifies UDP-galactopyranose mutase as an important protein in fungal cell wall biosynthesis.

Content Provider	Semantic Scholar
Author	Damveld, Robbert A. Franken, Angelique C. W. Arentshorst, Mark Punt, Peter J. Klis, F. M. Hondel, Cees Amjj Van Den Ram, Arthur F. J.
Copyright Year	2008
Abstract	To identify cell wall biosynthetic genes in filamentous fungi and thus potential targets for the discovery of new antifungals, we developed a novel screening method for cell wall mutants. It is based on our earlier observation that the Aspergillus niger agsA gene, which encodes a putative alpha-glucan synthase, is strongly induced in response to cell wall stress. By placing the agsA promoter region in front of a selectable marker, the acetamidase (amdS) gene of A. nidulans, we reasoned that cell wall mutants with a constitutively active cell wall stress response pathway could be identified by selecting mutants for growth on acetamide as the sole nitrogen source. For the genetic screen, a strain was constructed that contained two reporter genes controlled by the same promoter: the metabolic reporter gene PagsA-amdS and PagsA-H2B-GFP, which encodes a GFP-tagged nuclear protein. The primary screen yielded 161 mutants that were subjected to various cell wall-related secondary screens. Four calcofluor white-hypersensitive, osmotic-remediable thermosensitive mutants were selected for complementation analysis. Three mutants were complemented by the same gene, which encoded a protein with high sequence identity with eukaryotic UDP-galactopyranose mutases (UgmA). Our results indicate that galactofuranose formation is important for fungal cell wall biosynthesis and represents an attractive target for the development of antifungals.
File Format	PDF HTM / HTML
DOI	10.1534/genetics.107.073148
PubMed reference number	18245853
Journal	Medline
Volume Number	178
Issue Number	2
Alternate Webpage(s)	http://www.genetics.org/content/genetics/early/2008/02/03/genetics.107.073148.full.pdf
Alternate Webpage(s)	http://www.genetics.org/content/genetics/178/2/873.full.pdf
Alternate Webpage(s)	https://doi.org/10.1534/genetics.107.073148
Journal	Genetics
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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