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Kinetic Spectrophotometric Methods for the Determination of Amoxicillin in Pharmaceutical Preparation
| Content Provider | Semantic Scholar |
|---|---|
| Author | Al-Abachi, Mouayed Q. Sattar, Jwan A. Abdul |
| Copyright Year | 2013 |
| Abstract | Two simple, sensitive and economical kinetic spectrophotometric methods have been developed for the estimation of Amoxicillin in pure form and pharmaceutical preparations.The methods based on the oxidation of the studied drug by a known amount of potassium permanganate in alkaline medium and subsequent determination of manganate ion(Method A)and unconsumed potassium permanganate(Method B),at a suitable λmax=602 and 524 nm,respectively.The reacted oxidant was found to be corresponding to the drug content. The initial rate and fixed time (at 30 minutes) methods are utilized for construction of calibration graphs to determine the concentration of the studied drugs. The calibration graphs are linear in the concentration ranges of (0.5–9.0) μg.ml −1 and (1-11) μg.ml −1 using the initial rate and fixed time methods, respectively. The results are validated statistically and checked through recovery studies. The two methods have been successfully applied for the determination of the studied Amoxicillin in commercial dosage form. ينلاديصلا هرضحتسم يفو ةيقنلا وتروصب نيمسسكوملاا ريدقتل ةيكرح ةيفيط قرط يجابعلا مساق ديؤم * ، راتسلادبع يدع ناوج ءايميكلا مسق , مومعلا ةيمك , دادغب ةعماج . دادغب لا قا رع ءايميكلا مسق* , مومعلا ةيمك , ةيرصنتسملا ةعماجلا . دادغب .قا رعلا ةصلاخلا تيتطيوووسب تيتيكروووس تيوووتيحيط تيتييروووط ررووووط تيتيداوووصتقاو تيتوووساسس , تروووووصب تيموووسسكومكا ءاود ريدووويتل ووووساتوب دووووجوب ءاودوووومل دووووسككا وووااحت وووووما تيتييروووطلا دوووومتعت . ينكديووووصلا راروووصستسملا حوووواو ةووويينلا قوووووا موي راووونغنملا قووووا مويوووساتوبو ولوكا ةووييرطلا رانغنملا توووويا ريدووويت مووم ادوووااق طوووسو حووا دوووسكفم وووماعك راوونغنملا تيويم اكا تييجووملا تييلووطلا دونا ةويناملا ةييرطلا امكتوسم رويغلا 602 و 524 رتموناون ( دوجو دوقو اويعباتت ءاودوووولا ووووتسم ووووم وووسانتي دووووسكفملا وووماعلا تا س ةوووسارد متو. ةارووووسلا دووووعم حوووتييرط ةطووووساوب وووااحتلا ةوووويكر دونا روباملا تمزلاو ةيئادتبكا 30 ةوييقد ةويط لا دوعم تاوكو .تيتييروطلا يوكل وااحتمل وموصحلا لورو لا رويبمت موم وووولوكا ةوووييرطلا ماد توووساب ءاودووولا ريدوويتل 9.0-0.5 وووم.مارغوركيام 1 ةووويناملا ةوووييرطلا ماد توووسابو 111 م.مارغوركيام 1 يبطو, .ةينكديصلا رارصسسملا حا ءاودلا ريديت حا حاجنب تيتييرطلا ر 1-Introduction Amoxicillin(AMOX) is (2S,5R,6R)6{[(2R)-2-amino2-(4-hydroxyphenyl)acetyl]amino}3,3-dimethyl7-oxo4-thia1azabicyclo[3.2.0]heptane2-carboxylic acid and Al-Abachi and Abdul Sattar Iraqi Journal of Science,Vol 53,No 1,2012, Pp. 8-16 9 has a molecular formula C16H19N3O5S,whereas its chemicals structure is [1]: Amoxicillin is a penicillin antibiotic which is used to treat many different types of infections caused by bacteria, such as ear infections, bladder infections, pneumonia, gonorrhea, and E. coli or salmonella infection. Amoxicillin is also sometimes used together with another antibiotic called clarithromycin (Biaxin) to treat stomach ulcers caused by Helicobacter pylori infection [2].Several methods have been reported for determining this drug in pharmaceutical preparations,including Spectroscopy[3-8] SIA (Sequential Injection Analysis)[9], Voltametric [10],Flow InjectionChemiluminescence [11,12],FI Flow Injection)[13],UPLC-MS/MS(Ultra Performance Liquid Chromatography-Tandem Mass Spectroscopy)[14],SPE-LC/ESI-MSMS(Solid Phase Extraction-Liquid Chromatography-Electrospray-Tandem Mass Spectroscopy)[15] and Chromotographic[16,17] methods. The United states Pharmacopeia (USP) involved a high-performance liquid chromatography (HPLC) method for the determination of AMOX. in pharmaceutical preparation but this method requires large amount of high purity organic solvents and long system stabilization time [18]. Hence, it was planned to develop a sensitive and economically viable technique, like kinetic procedures. The literature is still poor in analytical procedures based on kinetics, especially for pharmaceuticals or biological fluids. However, some specific advantages in the application of kinetic methods can be expected such as, selectivity due to the measurement of the evolution of the absorbance with the time of the reaction instead of the measurement of a concrete absorbance value. Potassium permanganate has been frequently utilized in the field of pharmaceutical analysis. Many pharmaceutical compounds have been determined kinetically through this approach such as Telithromycin [19],cephalosporins[20,21],Tetracycline[22] Ramipri[23] . The aim of the present work was to study the reaction between Amoxicillin and alkaline potassium permanganate kinetically in an attempt to evaluate the drug in pharmaceutical preparation. The initial-rate and fixed-time methods were adopted after a full investigation |
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| Alternate Webpage(s) | https://www.iasj.net/iasj?aId=28893&func=fulltext |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
