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Determination of Naproxen in Pharmaceutical preparations by spectrophotometric and flow Injection – activated chemiluminescence methods
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ahmed, Abdul Majeed Khorsheed |
| Copyright Year | 2012 |
| Abstract | This study involves development of a simple spectrophotometric method and a new flow injection–activated chemiluminescence (FIA-Cl) for the determination of Naproxen (Nap) in Pharmaceutical preparations . Spectrophotometric method was based on the oxidation of the (Nap) with alkaline potassium permanganate, the reaction is followed spectrometrically by measuring the absorbance of (Nap) at 608 nm . The reaction time of oxidation of (48 min) method is adopted for determining the drug concentration. The calibration graph was linear in the range of (0.4-2.8)μg.ml -1 with a correlation coefficient of (0.9998), detection limit of (0.281) μg.ml -1 , molar absorption coefficient is 2.348×10 4 L/mol.cm and a relative standard deviation RSD% of (3.121.32%). The method of FIA-CL was based on the activation of luminol – cobalt – H2O2 chemiluminescence by (Nap). The linearity is (10-45) μg.ml -1 with detection limit of (5.5)μg.ml -1 , and correlation coefficient was (0.9999) n=6 and the relative standard deviation was (1.65-1.12%). The two methods were applied successfully to determine the content of (NaP) in pharmaceutical preparations with a recovery of 98.99% Introduction Naproxen (Lebbe et al. , 1997) is a proprionic acid derivate related to the arylacetic acid group of nonsteroidal anti-inflammatory drugs. it is antipyretic and analgesic effects were related to inhibition of cyclooxyenase. The chemical names for naproxen and naproxen sodium (Meijer & Molema, 1995) are (S) –6– methoxy –a– methyl –2– naphthalene acetic acid and (S) –6– methoxy –∞– methyl –2– naphthalene acetic acid, Sodium salt, respectively. Naproxen and naproxen sodium have the following structures, respectively: Journal of Kirkuk University – Scientific Studies, vol 6, No2, 2011 09 Naproxen (R= COOH) C14H14O3 , M.wt = 230.26 Naproxen Sodium (R=COONa) C14H13NaO3 , M.wt = 252.23 Various methods based on HPLC have been developed for determination of (Nap) as its metabolites and enatiomers in plasma, (Karidas et al., 1993; Singh et al. 1991), and in Synovial fluid and plasma (Andersen , 1992) (Blagbrough et al., 1992) and in human plasma and urine by means of gradient HPLC (Vree et al., 1992) (Hansen, 1992). Also this drug has been determind in a human serum by capillary electrophoresis (Helena et al. , 1992) with ultraviolet absorbance and Laser – induced fluorescence detection. Lotfi and Frida (2003) used HPLC with porous graphitic carbon (PGC) column and tetrahydro furan–methanol as the mobile phase to determine the (Nap) as degradation products (Baeyens et al., 1991) used microbore liquid chromatography (LC) with native fluorescence Detection for the determination of (NaP) in pharmaceutical preparations with 0.2μg.ml -1 detection limit. (Holzbecher, M.et al,1979) used an ultraviolet spectrophotometric procedure for the routine determination of (Nap) in serum. Also, another methods have determined (Nap) by titrimetry in tablets (Maheshwari et al., 2009), Capillary electrophoresis (Fillet et al., 1998), spectrofluorometry (Damiani et al., 2002), flow injection analysis (FIA) using UV-detector (sener et al., 2003), potentiometric titration (Hakan et al., 2008), Phosphorimetric (Inmaculada et al., 1999) and a rapid chemiluminescence method for the determination of (Nap) in pharmaceutical preparation based on the chemiluminescence reaction with Cerium(IV) in sulfuric acid medium( Campiglio, 1998; Hadir, 2008) used Synchronous Luminescence spectrometry to determine binary mixture of (NaP) and Difunisal. The present paper describes a spectrophotometric method for the determination of (NaP) in pharmaceutical preparations based on the oxidation with alkaline potassium permanganate. This study also Includes development of FIA-CL method . Experimental A) A spectrophotometer of type Hach /USA moldel DR 4000 Uv-Visible was used, with quartz cells of 1 cm width. B) FIA Chemiluminescence configuration which was outlined in figure (1) was used for the determination of (Nap). Journal of Kirkuk University – Scientific Studies, vol 6, No2, 2011 09 PPeristaltic pump VInjection valve FFlow cell dPhotomultiplier RRecorder WWaste solution . Fig. (1): Chemical reaction manifold used for the Flow-Injection Chemiluminescence determination of Nap. Reagents Reagents of analytical grade and distilled water were used through out the study ..Solutions were prepared by appropriate dissolution as shown in table (1). Table (1): preparation of some solutions Substance Molar Concentration (M) Dissolved weight (gm) Final Volume (ml) N a O H 1.0 4.000 100 Na2CO3 0.1 10.599 1000 Na2C2O4 0.1 13.39 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.iasj.net/iasj?aId=43120&func=fulltext |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
