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A neurotransmitter transporter encoded by the Drosophila inebriated gene.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Soehnge, Holly Huang, Xin Becker, Matthias Whitley, Penn P. Conover, Diana Stern, Marc-Henri |
| Copyright Year | 1996 |
| Abstract | Behavioral and electrophysiological studies on mutants defective in the Drosophila inebriated (ine) gene demonstrated increased excitability of the motor neuron. In this paper, we describe the cloning and sequence analysis of ine. Mutations in ine were localized on cloned DNA by restriction mapping and restriction fragment length polymorphism (RFLP) mapping of ine mutants. DNA from the ine region was then used to isolate an ine cDNA. In situ hybridization of ine transcripts to developing embryos revealed expression of this gene in several cell types, including the posterior hindgut, Malpighian tubules, anal plate, garland cells, and a subset of cells in the central nervous system. The ine cDNA contains an open reading frame of 658 amino acids with a high degree of sequence similarity to members of the Na+/Cl(-)-dependent neurotransmitter transporter family. Members of this family catalyze the rapid reuptake of neurotransmitters released into the synapse and thereby play key roles in controlling neuronal function. We conclude that ine mutations cause increased excitability of the Drosophila motor neuron by causing the defective reuptake of the substrate neurotransmitter of the ine transporter and thus overstimulation of the motor neuron by this neurotransmitter. From this observation comes a unique opportunity to perform a genetic dissection of the regulation of excitability of the Drosophila motor neuron. |
| Starting Page | 39 |
| Ending Page | 43 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/93/23/13262.full.pdf |
| PubMed reference number | 8917579v1 |
| Volume Number | 93 |
| Issue Number | 23 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acids Anus CNS disorder Clone Cells DNA, Complementary Embryo Motor Neurons Mutation Neurotransmitters Nucleic Acid Hybridization Open Reading Frames Reading Frames (Nucleotide Sequence) Restriction Mapping SLC6A2 protein, human Seminiferous tubule structure Sequence Analysis Sequence Analysis, Protein Transcript mutant |
| Content Type | Text |
| Resource Type | Article |
