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SERS detection of microRNA biomarkers for cancer diagnosis using gold-coated paramagnetic nanoparticles to capture SERS-active gold nanoparticles
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Hao Zhou, Chunhui Ying, Guoqing Zhou, Xiang-Dong Fu, Chaopeng Zhu, Yifeng Shen, Youqing |
| Copyright Year | 2017 |
| Abstract | In this paper, a magnetic-based, surface-enhanced Raman scattering (SERS) assay for detection of a cancer-related microRNA biomarker, miR-141, has been developed. The detection is based on hybridization-dependent recognition, in which the miR-141 target sequences were captured by complementary reporter and capture oligonucleotide probes conjugated to Raman-tagged gold nanoparticles (GNPs) and gold-coated paramagnetic nanoparticles (Au@MNPs) respectively. The resultant hybridization complexes, Raman-tagged GNPs/miR-141/Au@MNPs, are retrieved from solution by magnetic pull-down and concentrated within the focus of laser excitation. A signature spectrum for the Raman tag, 5,5′-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB), was observed in concentrated pellets and specific for the miR-141 sequences. The viability of SERS detection has been demonstrated in a microfluidic platform, in which the hybridizations containing dilutions of the miR-141 sequences yielded a reduction in the DSNB spectrum peaks' intensity. The limit of detection (LOD) is estimated to be 100 fM, which is 100-fold lower than the LOD of 10 pM previously reported in a similar magnetic-capture SERS detection of small oligonucleotides using nonplasmonic MNPs. These results indicate that the addition of Au shells to MNPs facilitates the formation of SERS-active junction regions (“hot spots”) with nearby Au contents within the magnetic concentrates, which substantially improves the SERS signal and, therefore, detection sensitivity. |
| Starting Page | 52782 |
| Ending Page | 52793 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/C7RA10918K |
| Volume Number | 7 |
| Alternate Webpage(s) | https://pubs.rsc.org/en/content/articlepdf/2017/ra/c7ra10918k |
| Alternate Webpage(s) | http://www.rsc.org/suppdata/c7/ra/c7ra10918k/c7ra10918k1.pdf |
| Alternate Webpage(s) | https://doi.org/10.1039/C7RA10918K |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
