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A PEGylation technology of L-asparaginase with monomethoxy polyethylene glycol-propionaldehyde.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Bochu Cao, Yang Chi, Shaoping Lou, Deshuai |
| Copyright Year | 2012 |
| Abstract | Polyethylene glycol (PEG) conjugation technology has been successfully applied to improve the performance of protein drugs. In this study, L-asparaginase was N-terminal site-specifically modified by alkylating PEG with monomethoxy polyethylene glycol-propionaldehyde (mPEG-ALD20000). The optimum reaction parameters were determined as pH 5.0, a molar ratio of mPEG-ALD2000 to L-asparaginase of 10:1, a reaction time of 16 h and temperature of 25 degrees C. PEG-L-asparaginase (PEG-L-ASNase) was isolated and purified with consecutive anion-exchange (XK, 16 x 20 cm, Q Sepharose FF) and gel-filtration (Tricorn, 10 x 600 cm, Sephacryl S-300 HR) chromatography, respectively. PEG-L-ASNase retained 43.5% of its activity and the N-terminal amino groups were modified to an extent of 3.67%. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.znaturforsch.com/ac/v67c/67c0312.pdf |
| Alternate Webpage(s) | http://www.znaturforsch.com/s67c/s67c0312.pdf |
| PubMed reference number | 22888537v1 |
| Volume Number | 67 |
| Issue Number | 5-6 |
| Journal | Zeitschrift fur Naturforschung. C, Journal of biosciences |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | ASPARAGINASE Anions Molar Ratio Polyethylene Glycols filtration pegaspargase peginterferon alfa-2b succinimidyl carbonate poly(ethylene glycol) monomethyl ether |
| Content Type | Text |
| Resource Type | Article |
