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Induction et prolifération de cals à partir de l’axe embryonnaire du Voandzou [ Vigna subterranea (L.) Verdc. Fabaceae] : effet de la segmentation de l’explant, des phytohormones, de la source de carbone et du génotype
| Content Provider | Semantic Scholar |
|---|---|
| Author | Konaté, Siriki Koné, M. C. Koffi, Edmond Kouablan Zouzou, Michel Kouadio, Justin Yatty |
| Copyright Year | 2013 |
| Abstract | Le Voandzou [ Vigna subterranea , (L.) Verdc.], occupe une place importante dans les strategies elaborees pour assurer la securite alimentaire en Afrique subsaharienne. Le developpement de systemes de regeneration in vitro de plantes, prealable au transfert de genes necessite l’etablissement de conditions optimales de la callogenese. Au cours du present travail, l’etude des facteurs influencant l’induction et la proliferation des cals chez le Voandzou a ete realisee. L’axe embryonnaire issu de graines matures a ete place sur le milieu de base de Murashige et Skoog (1962) additionne avec les vitamines B 5 et supplemente avec differentes concentrations et combinaisons de phytohormones. Apres quatre semaines de culture, les resultats ont montre que l’induction et la proliferation de cals ont ete favorisees avec le 2,4-D (0,5 mg/l). La partie basale de l’axe embryonnaire a ete la zone la plus favorable a la callogenese. La meilleure source de carbone a ete le saccharose a la concentration optimale de 84 mM. Les meilleurs taux d’induction (100 %) et de proliferation de cals (3) ont ete exprimes avec les ecotypes Ci2, Ci3, Ci4, Ci5, Ci6, Ci7, Ci10 et Ci21. Mots-cles : voandzou, axe embryonnaire, callogenese, phytohormones. Callus induction and proliferation from embryonic axis in Bambara groundnut [ Vigna subterranea (L.) Verdc. Fabaceae]: effect of explants section, plant growth regulators, carbon source and genotype Bambara groundnut [ Vigna subterranea (L.) Verdc. ] contributes to food security in sub-Saharan Africa. Development of efficient systems of in vitro plant regeneration a prerequisite to gene transfer requires establishment of optimal conditions for callus formation. In this work, factors influencing callus induction and proliferation in Bambara have been studied. Embryonic axis derived mature seeds were placed on Murashige and Skoog (1962) medium supplemented with vitamins B 5 (MSB 5 ), including different concentrations and combinations of plant growth regulators. After four weeks of culture, results showed that 2,4-D (0.5 mg/L) exhibited the best frequency and proliferation index of callus. Basal part of embryonic axis was the explants of choice for callus induction and proliferation. Sucrose at optimum concentration of 84 mM was favorable to the process of callus formation. Highest callus induction frequency (100 %) and proliferation index (3) were expressed by ecotypes Ci2, Ci3, Ci4, Ci5, Ci6, Ci7, Ci10 and Ci21. Keywords : bambara groundnut, embryonic axis, callogenesis, plant growth regulators. |
| Starting Page | 140 |
| Ending Page | 150 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| Volume Number | 9 |
| Alternate Webpage(s) | https://www.ajol.info/index.php/afsci/article/download/112040/101808 |
| Alternate Webpage(s) | http://www.afriquescience.info/docannexe.php?id=3033 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
