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Increased production of hydrogen peroxide by Lactobacillus delbrueckii subsp. bulgaricus upon aeration: involvement of an NADH oxidase in oxidative stress.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Marty-Teysset, C. Torre, Françoise De La Garel, Jonathan |
| Copyright Year | 2000 |
| Abstract | The growth of Lactobacillus delbrueckii subsp. bulgaricus (L. delbrueckii subsp. bulgaricus) on lactose was altered upon aerating the cultures by agitation. Aeration caused the bacteria to enter early into stationary phase, thus reducing markedly the biomass production but without modifying the maximum growth rate. The early entry into stationary phase of aerated cultures was probably related to the accumulation of hydrogen peroxide in the medium. Indeed, the concentration of hydrogen peroxide in aerated cultures was two to three times higher than in unaerated ones. Also, a similar shift from exponential to stationary phase could be induced in unaerated cultures by adding increasing concentrations of hydrogen peroxide. A significant fraction of the hydrogen peroxide produced by L. delbrueckii subsp. bulgaricus originated from the reduction of molecular oxygen by NADH catalyzed by an NADH:H(2)O(2) oxidase. The specific activity of this NADH oxidase was the same in aerated and unaerated cultures, suggesting that the amount of this enzyme was not directly regulated by oxygen. Aeration did not change the homolactic character of lactose fermentation by L. delbrueckii subsp. bulgaricus and most of the NADH was reoxidized by lactate dehydrogenase with pyruvate. This indicated that NADH oxidase had no (or a very small) energetic role and could be involved in eliminating oxygen. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://aem.asm.org/content/66/1/262.full.pdf |
| PubMed reference number | 10618234v1 |
| Volume Number | 66 |
| Issue Number | 1 |
| Journal | Applied and environmental microbiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Achromobacter denitrificans Biomass Excretory function Flavoproteins G1 to G0 transition Hydrogen Peroxide Lactate Dehydrogenase Lactic acid Lactobacillus sp Ab.IgG:ACnc:Pt:Ser:Qn Lactose NADH Oxidative Stress Oxygen Peroxides Pyruvates Subspecies bulgaricus exponential |
| Content Type | Text |
| Resource Type | Article |
