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PCR-DGGE analysis of denitrifying bacteria in a metallurgic wastewater treatment process.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Noda, Naohiro Yoshie, Sachiko Miyano, Takayuki Tsuneda, Satoshi Hirata, Akira Inamori, Yuhei |
| Copyright Year | 2002 |
| Abstract | The wastewater generated from the processes of recovering precious metals from industrial wastes contains high concentrations of acids such as nitric acid and of salts. Biological nitrogen removal from this wastewater was attempted by using a circulating bioreactor system equipped with an anoxic packed bed or an anoxic fluidized bed and an aerobic three-phase fluidized bed. The system was found to effectively remove nitrogen from the diluted wastewater (T-N; 1,000-4,000 mg litre(-1)). The microbial population structure of activated sludge in an anoxic reactor was analyzed by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA (rDNA) fragments. DGGE analysis under different operating conditions demonstrated the presence of some distinguishable bands in the separation pattern, which were most likely derived from many different species constituting the microbial communities. Furthermore, the population diversity varied in accordance with the nitrate-loading rate, water temperature and reactor condition. Some major DGGE bands were excised, reamplified and directly sequenced. It was revealed that the dominant population in the anoxic reactor were affiliated with the beta subclass of the class Proteobacteria. |
| File Format | PDF HTM / HTML |
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| Alternate Webpage(s) | https://watermark.silverchair.com/333.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAeUwggHhBgkqhkiG9w0BBwagggHSMIIBzgIBADCCAccGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMlnUGImorWstCZi_SAgEQgIIBmKDafoxlXG9oBJ5EQ5e-olIv6Y_RbKOraiGH1vgipRpEGGNG4_sQATMj3CjNaZ4xw2srHBDCnEQ502M4ruJH69cRBDtKhOMRU9NLFThM27Ru25ZTQitmBCA78xx8LMZnQNP6dah2Ye8cFjqn8RRTqgeXyaxglahwIDAoAMSvLETBCqQ28bP6alDf5Gh5mwzK1DIaVbcfWpzpqTgOmWWS98GEzy4iBtiKsk9NGfzAKJB-bFLthjSnCtd7HfbQqyrj-oV8L2ZED3PqdGZaKG1EiwdB_vyCVkqlEDWgLRUbd1xCT3b-hO-B0VocKAmZbj7AjQPiQbMWc4aPxSJWh29MgYU3sMdlbaliZdl0M2LDNvg36aXr8RX6VM2J1pzHRaXTpqrWTKMJrOAAIMTXZ5ud02ksPpBt6N-yMjqph9qDFJl8DyikJMImPMZqvD9IaMGDEOFQmXGswMk4l9ZTfAIygVKEQ3AkhdlJndD2SSLbd4i4InyqfXl47q9VknWLarQTb29aeHSCEJJVrEM1qRAxLo70k1PgepdLRA |
| PubMed reference number | 12216646v1 |
| Volume Number | 46 |
| Issue Number | 1-2 |
| Journal | Water science and technology : a journal of the International Association on Water Pollution Research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Attempt Bands Bioreactors Community DNA, Ribosomal Denaturing Gradient Gel Electrophoresis Gel Electrophoresis (lab technique) Metals Nitrate Nitric Acid Pack unit Reactor Device Component Salts subclass |
| Content Type | Text |
| Resource Type | Article |
