NDLI: IL-6 and IL-11 as Markers for Tumor Aggressiveness and Prognosis in Gastric Adenocarcinoma Patients without Mutations in Gp 130 Subunits

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IL-6 and IL-11 as Markers for Tumor Aggressiveness and Prognosis in Gastric Adenocarcinoma Patients without Mutations in Gp 130 Subunits

Content Provider	Semantic Scholar
Author	Necula, Laura Georgiana Chivu-Economescu, Mihaela Stănciulescu, Elena Luminiţa Bleotu, Coralia Dima, Simona Olimpia Alexiu, Irina Dumitru, Adriana Constantinescu, Gabriel E. Popescu, Irinel Eugen Diaconu, Carmen C.
Copyright Year	2012
Abstract	Background & Aims. A point mutation (gp130Y757F/ Y759F) was identified as being responsible for aberrant activation of gp130 in mice and associated with gastric adenocarcinoma induction. As a result, we investigated the possible role of key point mutations in Tyr from IL6ST exon 17 that encode for the catalytic domain of gp130, and of its respective activators (IL-6 family member cytokines) in human gastric cancer initiation and development. method. DNA, protein and plasma from 51 patients with gastric adenocarcinoma have been used in exploring gp130 status. We used sequencing analysis of IL6ST exon 17 in order to identify possible mutations that would lead to constitutive active forms of the receptor. The levels of gp130 activators (IL-6, IL-11, LIF) were analyzed by ELISA in plasma and mucosa of patients with gastric adenocarcinoma. Results. Sequencing analysis did not identify mutations in gp130 key positions (Y759, Y767, Y814, Y905 and Y915). An increased IL-6 and IL-11 level in gastric mucosa was observed, correlated with staging, indicating these cytokines as gp130 activators in tumor epithelial cell. Those variations were consistent with increased IL-6 level in plasma. Furthermore, IL-6, but not IL-11 showed a significant correlation with patient's survival time, suggesting that tissue and plasma concentration of IL-6 might be a marker of tumor aggressiveness with prognostic value. Conclusions. According to our results, no mutations were detected in gp130 key positions in human gastric adenocarcinoma samples. However, gp130 activation may occur due to the increased level of IL-6 and IL-11 cytokines detected that can become valuable biomarkers. *The first three authors equally contributed to this work.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.jgld.ro/2012/1/6.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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