Loading...
Please wait, while we are loading the content...
Similar Documents
COMPARACIÓN DE MÉTODOS CONVENCIONALES Y MOLECULARES PARA LA DETECCIÓN DE Giardia lamblia EN HECES HUMANAS
| Content Provider | Semantic Scholar |
|---|---|
| Author | Cardona, Edwin Castañeda, Silvia Morón Alvarez, María E. Pérez, Jorge Enrique Páez, Fredy Arvey Rivera Gartner, Germán Ariel López |
| Copyright Year | 2014 |
| Abstract | The protozoa of the genus Giardia represent one of the most common human parasites in the world and are among the main causes of gastrointestinal infections and diarrheal diseases in humans. Parasite detection is generally based on concentration and conventional microscopy methods, but these techniques have limitations due to their low sensitivity and specificity in diagnosis. In an attempt to improve the diagnostic methods, molecular techniques are emerging as a promising alternative. In this study 88 stool samples from patients of a company that provides health services (ASSBASALUD) in the city of Manizales (Caldas) were analyzed. In order to detect Giardia lamblia in stool, three different methods were compared using the positivity percent: conventional methods for concentration of the sample and microscopic observation, analysis by immunoassay (indirect ELISA) and finally the amplification of two nuclear gene sequences by PCR. Three positive samples were obtained by concentration and conventional microscopy, two by immunoassay and 26 by molecular techniques. The study suggests that routine diagnostic tests based on conventional microscopy and immunoassay have lower detection rate of this parasite and that this deficiency can be compensated by means of the implementation of molecular diagnostic methods based on PCR, as a complementary strategy to support the diagnosis of this protozoan. |
| Starting Page | 159 |
| Ending Page | 170 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| DOI | 10.17151/luaz.2014.38.10 |
| Alternate Webpage(s) | https://www.redalyc.org/pdf/3217/321731214010.pdf |
| Alternate Webpage(s) | http://www.scielo.org.co/pdf/luaz/n38/n38a10.pdf |
| Alternate Webpage(s) | https://doi.org/10.17151/luaz.2014.38.10 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
