NDLI: Antigenicity of the region encoded by exon8 of the human serine protease, HtrA2/Omi, is associated with its protein solubility

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Antigenicity of the region encoded by exon8 of the human serine protease, HtrA2/Omi, is associated with its protein solubility

Content Provider	Semantic Scholar
Author	Park, Hyo-Jin Choi, Ju-Youn Seong, Young-Mo Han, Chul Yoon, Sung-Joo Kim Kang, Seongman Rhim, Hyangshuk
Copyright Year	2004
Abstract	HtrA2/Omi, a mitochondrial serine protease, is pivotal in regulating apoptotic cell death. To determine the location of antigenic determinants in HtrA2/Omi, we expressed a series of the N-terminally truncated HtrA2/Omi as GST fusion proteins in E. coli. We assessed protein solubility and antigenic reactivity of various N-terminally truncated HtrA2/Omi proteins by binding to glutathione beads and immunoblot analyses, respectively. We identified that the region encoded by exon8 of HtrA2/Omi was expressed as a highly soluble form and contains an antigenic determinant specifically recognized by a polyclonal serum against HtrA2/Omi. Our data provide evidence that protein solubility of the specific region in target proteins may contribute to the antigenicity.
Starting Page	1597
Ending Page	1603
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1023/A:1025630013679
PubMed reference number	14584913
Journal	Medline
Volume Number	25
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1023/A:1025630013679
Alternate Webpage(s)	https://doi.org/10.1023/A%3A1025630013679
Journal	Biotechnology Letters
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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