NDLI: In vitro reassembly of the malolactic fermentation pathway of Leuconostoc oenos (Oenococcus oeni).

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In vitro reassembly of the malolactic fermentation pathway of Leuconostoc oenos (Oenococcus oeni).

Content Provider	Semantic Scholar
Author	Salema, M. Capucho, I. Poolman, Berend Romão, Maria Vitória San Dias, Martin Coachman
Copyright Year	1996
Abstract	The mechanism of metabolic energy generation by malolactic fermentation was studied with artificial membrane vesicles of Leuconostoc oenos (Oenococcus oeni). (Note that although L. oenos was recently reclassified as O. oeni [L. M. T. Dicks, F. Dellaglio, and M. D. Collins, Int. J. Syst. Bacteriol. 45:395-397, 1995], the old designation was kept in the present work.) Purified malolactic enzyme was entrapped in artificial membrane vesicles prepared from L. oenos cells able to transport L-malate. We show that the in vitro reconstituted system, including an electrogenic L-malate carrier and the decarboxylating malolactic enzyme, generated a proton motive force that was able to drive intravesicular accumulation of leucine.
File Format	PDF HTM / HTML
DOI	10.1128/jb.178.18.5537-5539.1996
PubMed reference number	8808948
Journal	Medline
Volume Number	178
Issue Number	18
Alternate Webpage(s)	http://www.rug.nl/research/portal/files/10214817/1996JBacteriolSalemaP.pdf
Alternate Webpage(s)	http://gbb.eldoc.ub.rug.nl/FILES/root/1996/JBacteriolSalema2/1996JBacteriolSalemaP.pdf
Alternate Webpage(s)	http://jb.asm.org/content/178/18/5537.full.pdf
Alternate Webpage(s)	https://core.ac.uk/download/pdf/12927492.pdf
Alternate Webpage(s)	https://doi.org/10.1128/jb.178.18.5537-5539.1996
Journal	Journal of bacteriology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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In vitro reassembly of the malolactic fermentation pathway of Leuconostoc oenos (Oenococcus oeni).

In vitro reassembly of the malolactic fermentation pathway of leuconostoc oenos (oenococcus oeni) (1996).

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In vitro reassembly of the malolactic fermentation pathway of Leuconostoc oenos (Oenococcus oeni).

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