NDLI: Reverse transcriptase and substrate dependence of the RNA hypermutagenesis reaction.

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Reverse transcriptase and substrate dependence of the RNA hypermutagenesis reaction.

Content Provider	Semantic Scholar
Author	Martínez, María De Los Ángeles Vartanian, J. P. Wain-Hobson, Simon
Copyright Year	1995
Abstract	G-->A hypermutation is a remarkable phenomenon resulting from retroviral reverse transcription in the presence of highly biased dNTP concentrations. Of the three reverse transcriptases (RTases) available, those of human immunodeficiency virus type 1 (HIV-1), avian myeloblastosis virus (AMV) and Moloney murine leukemia virus (MoMLV), the HIV-1 enzyme showed the greatest sensitivity to biased [dCTP]/[dTTP] ratios. The HIV-1 RTase was able to discriminate between dUTP, dITP and the four DNA precursors and was insensitive to pH. There was little preference for nucleotide contexts. A few exceptionally modified sequences were found presumably resulting from G-->A hypermutation and multiple strand transfer. This particular predilection of the HIV-1 and, by extrapolation, the lentiviral RTases towards G-->A hypermutation suggests that the phenomenon may have contributed to the remarkably elevated A content of these retroviral genomes.
File Format	PDF HTM / HTML
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PubMed reference number	7544458v1
Volume Number	23
Issue Number	14
Journal	Nucleic acids research
Language	English
Access Restriction	Open
Subject Keyword	Aves Avian Myeloblastosis Virus DNA-Directed RNA Polymerase Deoxyribonucleotide Triphosphate Dependence Drug Induced Thrombocytopenia Genome HIV Infections HIV-1 Immunologic Deficiency Syndromes Leukemia, B-Cell Moloney Leukemia Virus Moloney murine sarcoma virus Murine leukemia virus Nucleotides Retroviridae Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription deoxyuridine triphosphate
Content Type	Text
Resource Type	Article

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