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The viral protein 3CD induces an equilibrium between the viral protein and RNA synthesis in a cell-free system for poliovirus replication
| Content Provider | Semantic Scholar |
|---|---|
| Author | Verlinden, Y. Cuconati, Andrea Wimmer, Eckard Rombaut, Bart |
| Copyright Year | 2002 |
| Abstract | Summary. In a cell-free system derived from uninfected HeLa cells and programmed with poliovirus RNA, an unbalance between the different replication steps is observed. After programming, the vRNA is exclusively used as a template for viral translation. It takes hours before there is a switch from protein synthesis to RNA replication. This is probably the reason for the inefficient production of infectious virus (compared to the synthesis in infected cells). If, however, the cell-free system is programmed with vRNA and with a mRNA coding for the viral protein 3CD, an increase in vRNA synthesis is found early post-programming, resulting in a better balance of protein synthesis and RNA synthesis and an increased virus yield of at least 2 log10. These data show that a balance between translation, RNA replication and packaging is required to allow efficient viral proliferation.The virus yield could be increased by a further log10 by the addition of pirodavir (a capsid stabiliser) and 10% of rabbit reticulocyte lysate to the cell-free system. |
| Starting Page | 731 |
| Ending Page | 744 |
| Page Count | 14 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s007050200022 |
| PubMed reference number | 12038684 |
| Journal | Medline |
| Volume Number | 147 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/s007050200022 |
| Alternate Webpage(s) | https://doi.org/10.1007/s007050200022 |
| Journal | Archives of Virology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
