NDLI: Complementation of a yeast cell cycle mutant by an alfalfa cDNA encoding a protein kinase homologous to p34cdc2.

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Complementation of a yeast cell cycle mutant by an alfalfa cDNA encoding a protein kinase homologous to p34cdc2.

Content Provider	Semantic Scholar
Author	Hirt, Heribert Páy, Anikó Györgyey, János Bakó, László Németh, Károly Bögre, László Schweyen, Rudolf J. Heberle-Bors, Erwin Dudits, Denes Dr.
Copyright Year	1991
Abstract	The cdc2 protein kinase plays a central role in control of the eukaryotic cell cycle of animals and yeasts. We have isolated a cDNA clone (cdc2Ms) from alfalfa (Medicago sativa L.) that is homologous to the yeast cdc2/CDC28 genes. The encoded protein is 64% identical to the yeast and mammalian counterparts and shows all the prominent structural features known from these organisms. Antibody raised against a 16-amino acid synthetic peptide with crossreactivity against p34 proteins recognized a 34-kilodalton protein in extracts of alfalfa cells. When transferred into a fission yeast, the plant cdc2 homolog can complement a temperature-sensitive cdc2 mutant. Northern analysis revealed higher transcript levels in shoots and suspension cultures than in roots. In addition to the dominant transcript of 1.4 kilobases detected in the poly(A)+fraction, 2.5- and 1.2-kilobase transcripts were detected in total RNA preparations from shoots or somatic embryos. Suspension cultures that were induced to form somatic embryos by an auxin (2,4-dichlorophenoxyacetic acid) showed fluctuations in transcription pattern during the induction period and embryogenesis.
File Format	PDF HTM / HTML
DOI	10.1073/pnas.88.5.1636
PubMed reference number	2000373
Journal	Medline
Volume Number	88
Issue Number	5
Alternate Webpage(s)	http://www-urgv.versailles.inra.fr/pub/hirt/prj6_ComplementationYeastCellCycle.pdf
Alternate Webpage(s)	http://www.pnas.org/content/88/5/1636.full.pdf
Alternate Webpage(s)	https://doi.org/10.1073/pnas.88.5.1636
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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