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AND STARCH CHARACTERS OF GADUNG ( Dioscorea hispida Dennst . ) TUBER ACCESSION TIMOR LESTE , KALIMANTAN TENGAH
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nugroho, Laurentius Hartanto Estyaniyana, Anna |
| Abstract | Gadung (Dioscorea hispida Dennst.) known as a yam internationally is a medicinal plant. In Timor Islan, gadung is used as repellant of Rice bug (Leptocorisa oratorius F.). Moreover, in same places, gadung is used as an alternative food. The aims of the research were to know the toxicity, phytochemical contents and starch characters of gadung tuber which were collected from Timor Leste, Kalimantan Tengah, and Daerah Istimewa Yogyakarta (DIY). Dry tubers were prepared for toxicity and phtyochemical contents analyses and fresh tuber were used to observe the character of gadung starch. The starch characters were shape, diameters, and amounts of starch grains. Tubers were extracted by Soxhletation apparatus using chloroform and methanol. The toxicity of gadung tuber was done using Brine Shrimp Lethality Test (BSLT). Effect of toxicity from each extract was identified with the percentage of death larva and counted by probit analysis (LC50). Futhermore, the content of alkaloid, flavonoid, terpenoid of gadung tuber extract were detected using Thin Layer Chromatography. The starch characters were observed by stabbing the fresh tuber repeatedly with needles, was extracted viscous white fluid and attached to the glass slide and making cross section of tuber cortex, following by observation under light microscope. The results revealed that the highest toxicity was shown by chloroform extract of gadung accession from Timor Leste with the LC50 was 10 μg/mL. Extract of three accessions contained alkaloids, flavonoids and terpenoids. Volume 7, No. 1, Agustus 2015 55 Three accessions starch grain had same characters. The characters were round shape and aggregate of each grain. Among the three accessions, DIY accession presented the bigest size of starch diameters and much number of aggregate in each parencyma cells. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ejournal.litbang.depkes.go.id/index.php/toi/article/download/8839/804580458165 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |