Characterization of Asparagus officinalis hinokiresinol synthase(ABSTRACTS (MASTER THESIS FOR GRADUATE SCHOOL OF AGRICULTURE))

Content Provider	Semantic Scholar
Author	Yamamura, Masaomi
Copyright Year	2006
Abstract	S (MASTERlHESIS FORGRADUATE SCHOOL OFAGRIOJLTURE) Characterization ofAsparagus ofjicinalis hinokiresinol synthase Masaomi Yamamura Research Institute for Sustainable Humanosphere. Kyoto University Norlignans, which have a diphenylpentane (C6-C5-C6) structure, were found in coniferous trees and some monocotyledonous plants including Asparagus officinalis [1,2]. Their biosynthesis is of interest especially in relation to heartwood formation, a metabolic event specific to woody plants, because norlignans are deposited specifically in tree heartwood regions. This implies that the molecular mechanisms can be a clue to help us elucidate mechanisms for metabolic events specific to woody plants. Hinokiresinol is the simplest norlignan compound, it is therefore a good target for the study ofnorlignan biosynthesis. Hinokiresinol has two geometrical (E)and (Z)-isomers, which are phytoalexins of Cryptomeria japonica (Japanese cedar) and A. officinalis, respectively. Recently, biosynthetic processes and enzymatic formation of (E)and (Z)-hinokiresinols were demonstrated using C. japonica and A. officinalis cells, respectively [2-4]. In the present study, cDNAs encoding A. officinalis (Z)-hinokiresinol synthase were cloned for the first time and their recombinant proteins were characterized biochemically. References [1] T. Umezawa: Regul. Plant Growth Dev., 2001, 36, 57. [2] S. Suzuki, T. Umezawa, and M. Shimada: J Chem. Soc. Perkin., 1, 2001, 3252. [3] S. Suzuki, T. Nakatsubo, T. Umezawa, and M. Shimada: Chem. Commun., 2002, 1088. [4] S. Suzuki, M. Yamamura, T. Nakatsubo, T. Hattori, M. Shimada, and T. Umezawa: Chem. Commun., 2004,2838.
Starting Page	43
Ending Page	43
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Language	English
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Resource Type	Article