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Gold nanoparticles-based colorimetric assay for rapid detection of Salmonella species in food samples
| Content Provider | Semantic Scholar |
|---|---|
| Author | Prasad, Dinesh Shankaracharya Vidyarthi, Ambarish Sharan |
| Copyright Year | 2011 |
| Abstract | A rapid, sensitive and cost-effective method was developed for detection of foodborne pathogens, particularly Salmonella species. The method utilizes single stranded DNA (ssDNA) probes and non-functionalized gold nanoparticles to provide a colorimetric assay for the detection of PCR amplified DNA. Different food samples were tested with the PCR-based colorimetric assay parallel with the conventional culture method. The sensitivity and specificity of colorimetric assay was 89.15 and 99.04% respectively with reference to conventional culture method. The total time required to detect the Salmonella spp. present in food samples by the developed method is less than 8 h, including 6 h incubation. It was observed that the colorimetric assay was 10 times more sensitive than gel-based detection with the same concentration of DNA used for analysis. |
| Starting Page | 2227 |
| Ending Page | 2230 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s11274-011-0679-5 |
| Alternate Webpage(s) | http://www.ugcfrp.ac.in/images/userfiles/PAPER%20FRP.pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/s11274-011-0679-5 |
| Volume Number | 27 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
