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Inactive O6-methylguanine-DNA methyltransferase in human cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhukovskaya, N. E. Rydberg, Björn Karran, P. |
| Copyright Year | 1992 |
| Abstract | A plasmid encoding a recombinant human O6-methylguanine-DNA methyltransferase (MGMT) fused to a fragment of the bacteriophage lambda N protein has been constructed. The fusion protein retained methyltransferase activity when expressed at high levels in E.coli and was purified to essential homogeneity by a simple procedure. Antisera raised against the purified fusion protein recognized MGMT in western blots of extracts of human cells. For most cell lines, there was a quantitative relation between the amount of immunologically detectable MGMT protein and enzyme activity. However, four cell lines contained detectable MGMT protein despite having no measurable methyltransferase activity. Additionally, a HeLa line contained considerably more immunoreactive MGMT protein than could be accounted for by its methyltransferase activity. Thus, some cells contain significant amounts of inactive MGMT. Preliminary characterization of the inactive protein in HeLaS3 cells indicated that it has some properties in common with MGMT methylated at the active cysteine residue. |
| Starting Page | 1346 |
| Ending Page | 1348 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/20/22/10.1093_nar_20.22.6081/2/20-22-6081.pdf?Expires=1492403505&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=P2vroX4AdJs3BCKHvZ4-os2MxbDyMK8hjzzqngwJ~hoNvhCznqmwTaDiFd~8GY1AlagJt~LlmUynVh3p57xf~ZpX~XFV6W2Kws4-OhObi1O4h~f0fKE~g4CmYetlaF8Xmm1nlIgq3nBliUxCvC2XcaLzzwVNrIxZsPa1KDP8tMbymaPK5L~Sbuzx8mSFp6vHkwVqVjlyq-~axiHkBVUQPfZMbN2u6eIknOhd7rau6KOfNt9O0cAI0EztQ5emmAEoW-PjP3lD63Cd1cPQ5NEs0tQTdgUiMNyBseZKMUpr7OGIMrIuTJyKXAbi9xHS4WFQWcHyIJhdhTX-z~fQkCkFEw__ |
| PubMed reference number | 1461738v1 |
| Volume Number | 20 |
| Issue Number | 22 |
| Journal | Nucleic acids research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Bacteriophages Contain (action) Cultured Cell Line HeLa Cells Inactive - Biochemical Activity Level O(6)-Methylguanine-DNA Methyltransferase O-(6)-methylguanine Recombinants Western Blot chimeric protein enzyme activity methyltransferase activity |
| Content Type | Text |
| Resource Type | Article |
