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Development of near Infrared Coherent Anti-stokes Raman Scattering Microscopy Using Supercontinuum Generated from a Photonic Crystal Fiber
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kano, Hideaki Hamaguchi, Hiro-O |
| Copyright Year | 2004 |
| Abstract | Coherent anti-Stokes Raman scattering (CARS) microscopy has been attracting much attention as a new technique for vibrational spectroscopic imaging [1-3]. Since CARS is based on the thirdorder nonlinear optical process, it has an inherent three-dimensional sectioning capability without a pinhole as a confocal microscope. In addition, owing to the intense, uni-directional CARS signal, it does not require long data-acquisition time. In order to generate the CARS signal, the vibrational coherence must be created by a difference frequency of two lasers, namely pump and Stokes lasers. However, it requires complicated laser systems such as two synchronized Ti:sapphire oscillators [1,3] or an optical parametric amplifier with a Ti:sapphire regenerative amplifier system [2]. Although several reports have been made of single pulse CARS [4], the frequency bandwidth is so narrow that it does not cover the fingerprint region. In the present study, we use a supercontinuum generated from a photonic crystal fiber (PCF) for the Stokes pulse. The PCFs consist of the microstructured cladding region with a hexagonal array of air holes to guide light into the pure silica core. By injecting readily available low-power ultrashort pulses into the PCF, an ultrabroadband phase-coherent supercontinuum spanning more than one octave has been achieved [5]. Owing to the easily handling and compactness, the supercontinuum has been used in various applications including spectroscopy [6-8]. Figure 1 shows a schematic overview of the experimental setup. The light source is an unamplified Ti:sapphire laser (Coherent, Vitesse-800). Typical duration, pulse energy, repetition rate, and peak wavelength are 100fs, 12nJ, 80MHz, and 801nm, respectively. The laser pulses are divided into the pump and seed pulses for the supercontinuum generation. The seed pulse is coupled into the PCF (Crystal Fibre, NL-1.7-690 or Mitsubishi cable, LFR-141) with a microscope objective. For the CARS signal generation, the fundamental of the Ti:sapphire laser ( ) and the 1 ω |
| File Format | PDF HTM / HTML |
| DOI | 10.1071/sa0402094 |
| Alternate Webpage(s) | http://www.publish.csiro.au/SA/pdf/SA0402094 |
| Alternate Webpage(s) | https://doi.org/10.1071/sa0402094 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
