NDLI: Isolation, differentiation, and characterization of mesenchymal stem cells from human bone marrow

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Isolation, differentiation, and characterization of mesenchymal stem cells from human bone marrow

Content Provider	Semantic Scholar
Author	Baghaei, Kaveh Hashemi, Seyed Mohmoud Tokhanbigli, Samaneh Rad, Ali Asadi Assadzadeh-Aghdaei, Hamid Sharifian, Abdolhamid Zali, Mohammad Reza
Copyright Year	2017
Abstract	Aim We describe the minimum requirements and a simplified method for isolation and characterization of mesenchymal stem cells (MSCs) from human bone marrow. Background MSCs are well known adult stem cells present in many tissues such as adipocytes, chondrocytes, osteoblasts, and neurons. Many isolations and characterization methods have emerged to apply MSCs in the clinical applications, which many of them are expensive and time-consuming. Methods MSC isolation was carried out from human bone marrow, and cultured in defined medium. Cultures were maintained at 370C in a humidified atmosphere containing 5% CO2 for 48h. The medium was exchanged every 3-4 days. Adherent cells were characterized according to main criteria defined by ISCT, such as differentiation capability to adipocyte and osteoblast using specific differentiation mediums; also, flow cytometry verified MSC specific markers. Results Isolated MSCs had a fibroblastic-like appearance with adherent property to the culture plate. Differentiation function was proved with the formation of lipid drops and calcium oxalates on the differentiated MSCs and finally, purified MSCs from bone marrow were positive for cell surface markers, CD73, CD90, and CD105 while being negative for CD34 and CD45. Conclusion These findings confirm that the represented method is capable of isolating MSCs from bone marrow with proven results according to all minimum criteria defined by the International Society for Cellular Therapy (ISCT).
Starting Page	208
Ending Page	213
Page Count	6
File Format	PDF HTM / HTML
DOI	10.22037/ghfbb.v0i0.1089
PubMed reference number	29118937
Journal	Medline
Volume Number	10
Alternate Webpage(s)	http://journals.sbmu.ac.ir/ghfbb/index.php/ghfbb/article/download/1089/697
Alternate Webpage(s)	https://doi.org/10.22037/ghfbb.v0i0.1089
Journal	Gastroenterology and hepatology from bed to bench
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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