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Development of a method for the simultaneous measurement of serum testosterone, 17α-hydroxyprogesterone and androstenedione by liquid chromatography - tandem mass spectrometry (LC-MS/MS)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Henman, Michael Waller, Kate Warner, Janet Marshall, Gill |
| Copyright Year | 2014 |
| Abstract | Matrix: The method of Matuszewski3 was used to asses matrix effect. Six separate patient serum pools each fortified to 5 different concentrations (0, 0.5, 1, 2.5, 10 and 30 nmol/L) were graphed against the fortified target. Each of the slopes were compared using linear regression. A CV% of less than 5 suggests minimal matrix effect. Our results were testosterone 4.6%, 17OHP 4.7% and androstenedione 2.4%. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.aacb.asn.au/documents/item/4100 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |