NDLI: Glucose-6-phosphate dehydrogenase (G6PD) activity of human sperm.

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Glucose-6-phosphate dehydrogenase (G6PD) activity of human sperm.

Content Provider	Semantic Scholar
Author	Sarkar, Siddhartha Nelson, Arly J. Jones, Oliver W.
Copyright Year	1977
Abstract	Sperm from normal human donors and a G6PD-A- individual were examined for x-linked glucose-6-phosphate dehydrogenase activity and autosomally linked 6-phosphogluconate dehydrogenase activity. With the use of fluorescence microscopy, we divised a procedure to visualise in individual sperm cells the fluorescence of reduced coenzyme NADPH formed by each of the two enzymes in the presence of appropriate substrates. We found significant differences in the population distribution of sperm expressing each of the two activities, and the ratio of the two activities in sperm homogenate is very different from the one found in erythrocyte lysates. The possibility of haploid gene expression has been considered in interpreting these results.
Starting Page	301
Ending Page	305
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://jmg.bmj.com/content/jmedgenet/14/4/250.full.pdf
PubMed reference number	21966v1
Volume Number	14
Issue Number	4
Journal	Journal of medical genetics
Language	English
Access Restriction	Open
Subject Keyword	6-phosphogluconate Coenzymes Erythrocytes Fluorescence Gene Expression Haploidy Spermatozoa Cell Count X-linked inheritance glucose-6-phosphate dehydrogenase activity
Content Type	Text
Resource Type	Article

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