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JAM-2 siRNA intracellular delivery and real-time imaging by proton-sponge coated quantum dots.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Qi, Lifeng Shao, Weijun Shi, Donglu |
| Copyright Year | 2013 |
| Abstract | In this study, proton-sponge coated quantum dots were prepared by using amphipol PMAL, grafted with polyethylenimine (PEI) as an encapsulation polymer. The QD-PMAL-PEI nanoparticles showed low cytotoxicity and superior gene silencing efficiency in serum-containing medium against junctional adhesion molecule-2 (JAM-2), which is over-expressed in glioma cells. Confocal microscopic analysis showed efficient siRNA intracellular release. In particular, QD-mediated JAM-2 knockdown was reported for the first time to facilitate inhibition of glioma cell migration. Furthermore, the Notch pathway served as the target for the JAM-2 gene function, confirmed by downregulation of its downstream genes HES1 and HES5. The unique proton-sponge coated QDs can serve as multifunctional siRNA carriers for efficient gene silencing and real-time intracellular imaging, and provide a base for design of novel efficient siRNA delivery carriers with high biocompatibility. |
| Starting Page | 654 |
| Ending Page | 660 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/C2TB00027J |
| PubMed reference number | 32260769 |
| Journal | Medline |
| Volume Number | 1 |
| Issue Number | 5 |
| Alternate Webpage(s) | http://homepages.uc.edu/~shid/publications/PDFfiles/JMCB.pdf |
| Alternate Webpage(s) | https://doi.org/10.1039/C2TB00027J |
| Journal | Journal of materials chemistry. B |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
