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Identificação e análise filogenética de espécies do gênero Sporothrix isoladas em área endêmica de esporotricose no Estado do Rio de Janeiro
| Content Provider | Semantic Scholar |
|---|---|
| Author | Oliveira, Manoel Marques Evangelista |
| Copyright Year | 2009 |
| Abstract | Sporothrix schenckii is the species responsible for sporotrichosis, an important subcutaneous mycosis with a worldwide distri bution, and very frequent in Latin America, and it has been reported as endemic in Rio de Janeiro, Brazil. Recent molecular studies have demonstrated that this species constitutes a complex of numerous phylogenetic species, which were phenotipically characteri zed using different culture media, growth rates at different temperatur es, and nutritional tests as we ll comparison calmodulin genes and it was proposed four new species Sporothrix brasiliensis , Sporothrix globosa , Sporothrix mexicana and Sporothrix luriei . And just 25 isolates from the endemic occurring in Rio de Janeiro were included in this previous stu dy. We have studied a total of 248 Sporothrix isolates from this endemic obtaine d during the period of 1998 and 2008. The phenotypic characterization was based on th e morphology of conidia, growth rates at 30oC and 37 oC, ability to grow at 37 oC, and auxonographic method. A ccording to the key features for species differentiation, 206 isolates (83.1%) were characterized as S. brasiliensis , one as S. mexicana (0.4%), 15 (6.0%) as S. schenckii and in 26 isolates (10.5%) the species was not differe ntiated being classified as Sporothrix spp. Among 8 (31%) of these unidentified isolates, molecular methodology, by sequence analysis of on protein coding locus (calmodulin) was perfor med, revealing the presence of one major clade, grouping 7 of these isolates with S. brasiliensis, representing this phylogenetic species. The analysis in genotype level should be used as complemental tool in the identification of species of the Sporothrix complex. The correlation among molecular data and phenotipe is fundamental in the identification of species Sporothrix complex , making possible the implementation of the polyphasic taxonomy |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.arca.fiocruz.br/bitstream/icict/9241/1/manoel_oliveira_ipec_mest_2009.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
