NDLI: In vitro propagation and quality evaluation of long-term micro-propagated and conventionally grown Fagopyrum dibotrys Hara mutant, an important medicinal plant

Please wait, while we are loading the content...

In vitro propagation and quality evaluation of long-term micro-propagated and conventionally grown Fagopyrum dibotrys Hara mutant, an important medicinal plant

Content Provider	Semantic Scholar
Author	Chen, Caixia Lan, Jinxu Cui, Siran Li, Ailian
Copyright Year	2012
Abstract	Fagopyrum dibotrys (D. Don) Hara is an important plant species of medicinal value. An efficient protocol for in vitro propagation of an F. dibotrys mutant that contains a higher level of (-)-epicatechin was developed. Adventitious buds were directly regenerated from stem nodal explants cultured in Murashige-Skoog (MS) basal medium containing 3% sucrose and appropriate combinations of plant growth regulators. In the presence of 2.0 mg/l 6-benzylaminopurine (6-BA), 0.5 mg /l thidiazuron (N-phenyl-N’-1,2,3-thidiazol-5-urea) (TDZ), and 0.2 mg/l naphthaleneacetic acid (NAA), the frequency of organogenesis was 90%, with a mean of 9.09 shoots per explants. The greatest rooting frequency, root number and length were attained using 1/2 MS medium containing 0.5 mg/l NAA and 2% sucrose. After two years of in vitro propagation and conservation, the regenerating plantlets were successfully transplanted into the field, and none showed morphological variations after one year of field growth. The regenerated rhizomes were assessed for drug-yielding potential through evaluation of (-)-epicatechin contents by HPLC, and then compared with those grown conventionally. The genetic basis of the stable drug yielding potential of the micro-propagated perennial buckwheat was revealed by random amplified polymorphic DNA-based profiling. Key words: Fagopyrum dibotrys, Shoot organogenesis, (-)-Epicatechin content, High performance Liquid Chromatography (HPLC), RAPD analysis.
Starting Page	3003
Ending Page	3012
Page Count	10
File Format	PDF HTM / HTML
DOI	10.5897/JMPR11.1508
Volume Number	6
Alternate Webpage(s)	https://academicjournals.org/article/article1380872115_Chen%20et%20al.pdf
Alternate Webpage(s)	https://doi.org/10.5897/JMPR11.1508
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in