NMR Structure Determination of Saccharose and Raffinose by Means of Homo- and Heteronuclear Dipolar Couplings

Content Provider	Semantic Scholar
Author	Neubauer, Heike Meiler, Jens Peti, Wolfgang Griesinger, Christian
Copyright Year	2001
Abstract	Residual dipolar couplings have dramatically improved the accuracy and precision of high-resolution NMR structures during the last years. This was first demonstrated for proteins. In this article, we describe, with raffinose and saccharose as examples, that dipolar couplings improve the precision of structures of carbohydrates for which usually very few structural parameters are available. The relative orientation as well as the dynamics of the monosaccharide moieties with respect to each other can be determined with the help of 13C,1H and 1H,1H dipolar couplings, which can easily be measured. Significant differences between the solution and the X-ray crystal structure exist. These results indicate that residual dipolar-coupling data may provide a more complete and dynamic model of carbohydrates in particular, and small molecules in general.
Starting Page	243
Ending Page	258
Page Count	16
File Format	PDF HTM / HTML
Volume Number	84
Alternate Webpage(s)	http://www.meilerlab.org/index.php/publications/showPublication/pub_id/34
Alternate Webpage(s)	https://doi.org/10.1002/1522-2675%2820010131%2984%3A1%3C243%3A%3AAID-HLCA243%3E3.0.CO%3B2-F
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article