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Trehalose-6-phosphate synthase/phosphatase regulates cell shape and plant architecture in Arabidopsis.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hicks, Glenn R. Choi, Yoon Gi Carter, David G. |
| Copyright Year | 2008 |
| Abstract | The vacuole occupies most of the volume of plant cells; thus, the tonoplast marker delta-tonoplast intrinsic protein-green fluorescent protein delineates cell shape, for example, in epidermis. This permits rapid identification of mutants. Using this strategy, we identified the cell shape phenotype-1 (csp-1) mutant in Arabidopsis thaliana. Beyond an absence of lobes in pavement cells, phenotypes included reduced trichome branching, altered leaf serration and stem branching, and increased stomatal density. This result from a point mutation in AtTPS6 encoding a conserved amino-terminal domain, thought to catalyze trehalose-6-phosphate synthesis and a carboxy-terminal phosphatase domain, is catalyzing a two-step conversion to trehalose. Expression of AtTPS6 in the Saccharomyces cerevisiae mutants tps1 (encoding a synthase domain) and tps2 (encoding synthase and phosphatase domains) indicates that AtTPS6 is an active trehalose synthase. AtTPS6 fully complemented defects in csp-1. Mutations in class I genes (AtTPS1-AtTPS4) indicate a role in regulating starch storage, resistance to drought, and inflorescence architecture. Class II genes (AtTPS5-AtTPS11) encode multifunctional enzymes having synthase and phosphatase activity. We show that class II AtTPS6 regulates plant architecture, shape of epidermal pavement cells, and branching of trichomes. Thus, beyond a role in development, we demonstrate that the class II gene AtTPS6 is important for controlling cellular morphogenesis. |
| Starting Page | 97 |
| Ending Page | 107 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.plantphysiol.org/content/plantphysiol/146/1/97.full.pdf |
| PubMed reference number | 17981987v1 |
| Volume Number | 146 |
| Issue Number | 1 |
| Journal | Plant physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Carboxyl Group Droughts Epidermis Green Fluorescent Proteins Inflorescence License Phenotype Plant Cells Point Mutation Starch Trehalose Trichomes cell morphogenesis lobe mutant phosphoric monoester hydrolase activity plant-type vacuole membrane trehalose-6-phosphate trichome branching |
| Content Type | Text |
| Resource Type | Article |
