NDLI: A recombination based method to rapidly assess specificity of two-hybrid clones in yeast.

Content Provider	Semantic Scholar
Author	Petermann, Robert Mossier, Birgit M. Kovar, Heinrich
Copyright Year	1998
Abstract	The yeast two-hybrid system is frequently used to identify protein-protein interactions. Confirming the specificity of candidate clones requires separation and isolation of yeast plasmids, propagation in bacteria and testing combinations of DNA-binding and activation domain hybrids in yeast. In order to simplify this procedure, we developed a rapid method based on PCR amplification of library insert DNAs and in vivo cloning into the activation domain hybrid vector. Reporter gene activity is assayed in parallel for combinations with different DNA-binding domain hybrids. Further characterization of inserts does not require plasmid isolation and intermediate hosts.
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Alternate Webpage(s)	https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/26/9/10.1093_nar_26.9.2252/2/26-9-2252.pdf?Expires=1492911221&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=FJB6VRN2WuyP~WiHmRURhE~FhYPbhY4WNRzZe2qrF0jTrrgGO1owoWHQ51XsQbK87kEj0xLdhm4KnQW0~DV3ujfuG38otasvkCTuXJsZ7x6SD312~dP46tSHRxb9lgDcI92FEHvtwk-06GZSQyJdViHuK8Irn74y5mO0sFRZa2O-cCsx-YKXighC0eVeBtSs5I1L7PzcfYm44Yat9IH5THZU4kCFYMdKLnEdVBpzUm6enPoGqRruKf2wjyfDi5lY2rpVPphl8QIoxhNSmhZ7YDTqjyuiBw8qk0eMShxDPljOEiqJjOeKRQgUPZROvEnMO42gOtx8ypoyLtWa5vm5Jw__
PubMed reference number	9547290v1
Volume Number	26
Issue Number	9
Journal	Nucleic acids research
Language	English
Access Restriction	Open
Subject Keyword	Hybrids Plasmids Recombination, Genetic Two-Hybrid System Techniques protein protein interaction
Content Type	Text
Resource Type	Article

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