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Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Is Involved in Adult Mouse Hippocampal Neurogenesis After Stroke
| Content Provider | Semantic Scholar |
|---|---|
| Author | Matsumoto, Minako Nakamachi, Tomoya Watanabe, Jun Sugiyama, Koichi Ohtaki, Hirokazu Murai, Norimitsu Sasaki, Shun Xu, Zhifang Hashimoto, Hitoshi Seki, Tamotsu Miyazaki, Akira Shioda, Seiji |
| Copyright Year | 2016 |
| Abstract | In the subgranular zone (SGZ) of the hippocampus, neurogenesis persists throughout life and is upregulated following ischemia. Accumulating evidence suggests that enhanced neurogenesis stimulated by ischemic injury contributes to recovery after stroke. However, the mechanisms underlying the upregulation of neurogenesis are unclear. We have demonstrated that a neuropeptide, pituitary adenylate cyclase-activating polypeptide (PACAP), exerts a wide range of effects on neural stem cells (NSCs) during neural development. Here, we examined the effects of endogenous and exogenous PACAP in adult NSCs of the SGZ. Immunostaining showed expression of the PACAP receptor PAC1R in nestin-positive NSCs of adult naive mice. PACAP injection into the lateral ventricle increased bromodeoxyuridine (BrdU)-positive proliferative cells in the SGZ. These data suggest that PACAP promoted the proliferation of NSCs. In global ischemia model mice, the number of BrdU-positive cells was increased in wild-type mice but not in PACAP heterozygous knockout mice. The BrdU-positive cells that increased in number after ischemia were immunopositive for SOX2, a marker of NSCs, and differentiated into NeuN-positive mature neurons at 4 weeks after ischemia. These findings suggest that PACAP contributes to the proliferation of NSCs and may be associated with recovery after brain injury. |
| Starting Page | 270 |
| Ending Page | 279 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s12031-016-0731-x |
| PubMed reference number | 26910758 |
| Journal | Medline |
| Volume Number | 59 |
| Alternate Webpage(s) | https://lilitory.showa-u.ac.jp/index.php?action=pages_view_main&active_action=repository_action_common_download&attribute_id=39&block_id=20&file_no=3&item_id=4317&item_no=1&page_id=15 |
| Alternate Webpage(s) | https://doi.org/10.1007/s12031-016-0731-x |
| Journal | Journal of Molecular Neuroscience |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
