NDLI: Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL-60 cells

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Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL-60 cells

Content Provider	Semantic Scholar
Author	Wei, Cheng Chen, Chuanrong Cheng, Yuxin Wang, Yu He, Yang Yang, Zhiming Ji, Zhaoning
Copyright Year	2018
Abstract	Ailanthone, which is extracted from the traditional Chinese medicinal plant Ailanthus altissima, has been thoroughly demonstrated to have anti-tumor, anti-HIV, anti-inflammatory, anti-malarial, anti-allergic and anti-microbial activities. However, the anti-proliferative effects of ailanthone on HL-60 cells and potential mechanisms underlying those effects have not been reported. In the present study, we demonstrated the potent cytotoxicity of ailanthone against HL-60 cells. Annexin V-APC/7-ADD staining assay indicated that ailanthone increased the number of apoptotic cells in a dose-dependent manner. PI staining showed that ailanthone increased the percentage of G0/G1-phase cells in a dose-dependent manner. Acridine orange staining suggested that ailanthone induced the formation of acidic vesicular organelles in HL-60 cells and pretreatment with BaF-A1 could attenuate this process. Western blotting showed that ailanthone up-regulated the protein expression levels of beclin-1 and LC3-II and down-regulated those of LC3-I and p62 in a dose-dependent manner. Use of BaF-A1 showed that the anti-proliferative effects of ailanthone on HL-60 cells may be partly attributable to the induction of autophagy-mediated apoptosis by MTT assay and annexin V-APC/7-ADD staining assay.
Starting Page	3569
Ending Page	3576
Page Count	8
File Format	PDF HTM / HTML
DOI	10.3892/ol.2018.9101
Alternate Webpage(s)	https://www.spandidos-publications.com/ol/16/3/3569/download
PubMed reference number	30127963
Alternate Webpage(s)	https://doi.org/10.3892/ol.2018.9101
Journal	Medline
Volume Number	16
Journal	Oncology letters
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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