NDLI: Fracionamento e caracterização parcial de constituintes químicos do extrato bruto de Pouteria ramiflora (Mart.) Radlk (Sapotaceae) biomonitorados pela inibição in vitro da atividade da alfa-Amilase Salivar Humana (HSA)

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Fracionamento e caracterização parcial de constituintes químicos do extrato bruto de Pouteria ramiflora (Mart.) Radlk (Sapotaceae) biomonitorados pela inibição in vitro da atividade da alfa-Amilase Salivar Humana (HSA)

Content Provider	Semantic Scholar
Author	Moura, Vilma Lucia
Copyright Year	2008
Abstract	Pouteria ramiflora (Mart.) Radlk is a plant specie of the Sapotaceae family , known popularly in Brazil as massaranduba, cariola, rooster grain and pitomba. The fractionation, isolation and identification of bioactive compounds from the crude extract of P. ramiflora were monitored by the inhibition of human salivary alpha-amylase (HSA) activity by in vitro microplate assay using enzymatic kinetic method with GalG2CNP as substrate. Probing these HSA activity with different crude extracts of P.ramiflora revealed that a leaf hydroalcoolic extract with higher inhibition property (64%). The fractionation of this leaf extract were conducted monitoring fractions through thin layer chromatography (TLC) analyses, liquid chromatography of high efficiency (HPLC) purification and inhibitory HSA activity. The TLC plates revealed the presence of flavonoids and tannins. Those factions showing higher inhibition activity were investigated by analytic and semi-preparative HPLC allowing the obtention of an isolated compound in one fraction and mixture of not identified flavonoids in the other one. The flavonoids presented in both fractions were less effective to inhibit HSA activity (15 and 20% respectively). This finding suggest an inhibitory effect of the P. ramiflora crude extract associated to a phytocomplex that could be lost during its phytochemistry fractionation.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://repositorio.ufu.br/bitstream/123456789/21394/1/FracionamentoCaracterizacaoParcial.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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