Loading...
Please wait, while we are loading the content...
Similar Documents
Cellular and humoral immune responses to alphavirus replicon vaccines expressing cytomegalovirus pp65, IE1, and gB proteins.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Reap, Elizabeth A. Dryga, Sergey A. Morris, J. A. G. Rivers, Bryan A. Norberg, Pamela K. Olmsted, Robert A. Chulay, Jeffrey David |
| Copyright Year | 2007 |
| Abstract | Development of vaccines against cytomegalovirus (CMV) is an important public health priority. We used a propagation-defective, single-cycle RNA replicon vector system derived from an attenuated strain of an alphavirus, Venezuelan equine encephalitis virus, to produce virus-like replicon particles (VRP) expressing various combinations of pp65, IE1, or gB proteins of human CMV. Protein expression in VRP-infected cells was highest with single-promoter replicons expressing pp65, IE1, a pp65/IE1 fusion protein, or the extracellular domain of gB and with double-promoter replicons expressing pp65 and IE1. Protein expression was lower with double- and triple-promoter replicons expressing gB, especially the full-length form of gB. BALB/c mice immunized with VRP expressing gB developed high titers of neutralizing antibody to CMV, and mice immunized with VRP expressing pp65, IE1, or a pp65/IE1 fusion protein developed robust antigen-specific T-cell responses as measured by gamma interferon enzyme-linked immunospot assay. Three overlapping immunodominant pp65 peptides contained a nine-amino-acid sequence (LGPISGHVL) that matches the consensus binding motif for a major histocompatibility complex H2-D(d) T-cell epitope. These data provide the basis for further development and clinical evaluation of an alphavirus replicon vaccine for CMV expressing the pp65, IE1, and gB proteins. |
| File Format | PDF HTM / HTML |
| DOI | 10.1128/CVI.00037-07 |
| PubMed reference number | 17442845 |
| Journal | Medline |
| Volume Number | 14 |
| Issue Number | 6 |
| Alternate Webpage(s) | http://cvi.asm.org/content/14/6/748.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1128/CVI.00037-07 |
| Journal | Clinical and vaccine immunology : CVI |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
