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Simultaneous spectrofluorimetric determination of (acetyl)salicylic acid, codeine and pyridoxine in pharmaceutical preparations using partial least-squares multivariate calibration.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Martos, N. R. Navalón, Alberto Payá, I. De Orbe Vallvey, Luis Fermín Capitán |
| Copyright Year | 2000 |
| Abstract | A partial least-squares calibration (PLS) method for the simultaneous spectrofluorimetric determination of salicylic acid (SA), codeine (CO) and pyridoxine (PY) is proposed. The determination of SA, CO, and PY has been carried out in mixtures of up to three components by recording the emission fluorescence spectra between 300 and 500 nm (lambda(exc) = 220 nm). Due to the fact of the strong spectral overlap among the excitation and also among the emission spectra of these compounds, a previous separation should be carried out in order to determine them by conventional spectrofluorimetric methodologies. Here, a full-spectrum multivariate calibration PLS method is developed. The experimental calibration matrix was constructed with 14 samples. The concentration ranges considered were 0.1-2.0 (SA), 0.25-3.0 (CO) and 0.10-2.0 (PY) mg x l(-1). The optimum number of factors was selected by using the cross-validation method. The method also allows the simultaneous determination of acetylsalicylic acid (ASA), CO and PY by previous alkaline hydrolysis of ASA to SA. To check the accuracy of the proposed method, it was applied to the determination of these compounds in synthetic mixtures and in pharmaceuticals. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://hera.ugr.es/doi/14999365.pdf |
| PubMed reference number | 11022909v1 |
| Volume Number | 23 |
| Issue Number | 5 |
| Journal | Journal of pharmaceutical and biomedical analysis |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Aspirin Calibration Codeine Cross Reactions Dosage Forms Epilepsies, Partial Excitation Fluorescence Papillon-Lefevre Disease Salicylic Acid Thioctic Acid analyte mixture pyridoxine |
| Content Type | Text |
| Resource Type | Article |
