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Extraction of DNA from eggs of grape phylloxera ( Daktulosphaira vitifoliae FITCH) for use in RAPD testing
| Content Provider | Semantic Scholar |
|---|---|
| Author | Walker, Melissa A. |
| Copyright Year | 2015 |
| Abstract | Three different extraction methods for preparing DNA from single grape phylloxera ( Daktulosphaira vitifoliae ) eggs for use in PCR-RAPD assay were compared. Pulverization in a glass micro-grinder produced much more clear and consistent RAPD results than when the egg DNA was extracted with a Chelex lysis or a proteinase-K + lysis. Multiple single egg extractions from the same parthenogenetic colony were done with all three methods; no RAPD assay differences were detected. This technique will allow evaluation of the roles population diversity, mutation rates, and reproductive mechanisms play in the Vitis /phylloxera interaction. |
| Starting Page | 87 |
| Ending Page | 89 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| Volume Number | 35 |
| Alternate Webpage(s) | https://ojs.openagrar.de/index.php/VITIS/article/download/4915/4707 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |