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Ultrasensitive colorimetric carcinoembryonic antigen biosensor based on hyperbranched rolling circle amplification.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Liang, Kai Zhai, Shuiting Zhang, Zhidong Fu, Xiaoyang Shao, Jingwei Lin, Zhenyu Chen, Guo-Nan |
| Copyright Year | 2014 |
| Abstract | In this study, a hyperbranched rolling circle amplification (HRCA)-based colorimetric biosensor for carcinoembryonic antigen (CEA) is developed with high sensitivity and specificity. A CEA aptamer can bind with its target (CEA) to form a complex due to their high affinity, and the introduced CDNA cannot hybridize with the aptamer. Thus, free CDNA can propagate the HRCA reaction to form a large number of single-stranded DNA (ss-DNA). ss-DNA can be easily adsorbed onto AuNPs and prevent salt-induced AuNPs aggregation, which causes the change in the color of the system. It is found that the absorbance intensity ratio (A520/A660) has a linear relationship with the concentration of the target in the range of 5 pM-0.5 nM, and the detection limit is as low as 2 pM (S/N = 3). |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/c4an00417e |
| PubMed reference number | 24996292 |
| Journal | Medline |
| Volume Number | 139 |
| Issue Number | 17 |
| Alternate Webpage(s) | http://pubs.rsc.org/en/content/getauthorversionpdf/C4AN00417E |
| Alternate Webpage(s) | https://doi.org/10.1039/c4an00417e |
| Journal | The Analyst |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
