Loading...
Please wait, while we are loading the content...
Similar Documents
β-amyloid peptide fragment 31–35 induces apoptosis in cultured cortical neurons
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dou, Yan-Ye Qiao, Zhuhui |
| Copyright Year | 1999 |
| Abstract | Abstract A synthetic fragment 31–35 of β-amyloid peptide was used in cultured cortical neurons to examine whether this smaller sequence could trigger apoptotic degeneration in vitro by using morphological, biochemical and flow-cytometric examinations. The results showed that: (i) neurons treated with fragment 31–35 of β-amyloid peptide exhibited membrane blebbing, compaction of nuclear chromatin, nuclear shrinkage and nuclear fragmentation; (ii) a typical DNA ladder was revealed by agarose gel electrophoresis following fragment 31–35 of β-amyloid peptide exposure; (iii) the internucleosome DNA fragmentation was also detected by flow-cytometric examination following fragment 31–35 of β-amyloid peptide exposure; and (iv) the DNA fragmentation induced by fragment 31–35 of β-amyloid peptide in the above two examinations could be blocked by co-treatment with aurintricarboxylic acid or actinomycin D. It is suggested that fragment 31–35 of the β-amyloid peptide may be a shorter sequence of β-amyloid peptide responsible for triggering an apoptotic process in cultured neurons. |
| Starting Page | 177 |
| Ending Page | 184 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0306-4522(98)00727-1 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0306452298007271 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0306452298007271?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/S0306-4522%2898%2900727-1 |
| Volume Number | 92 |
| Journal | Neuroscience |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
