Catecholaminergic cell lines from the brain and adrenal glands of tyrosine hydroxylase-SV40 T antigen transgenic mice.

Content Provider	Semantic Scholar
Author	Suri, Crystal
Copyright Year	1993
Abstract	Brain (CATH.a) and adrenal (PATH.1 and PATH.2) cell lines have been established that synthesize abundant dopamine and norepinephrine and express the appropriate catecholaminergic biosynthetic enzymes, tyrosine hydroxylase (TH) and dopamine beta-hydroxylase. The lines were derived from TH-positive tumors in transgenic mice carrying the SV40 T antigen oncogene under the transcriptional control of 773 base pairs of 5' flanking sequences from the rat TH gene. Although the lines continue to express T antigen, they exhibit neuronal properties such as neurofilaments and synaptophysin and lack glial intermediate filaments. Although in vivo TH is only expressed in postmitotic neurons in the CNS, the CATH.a line demonstrates that TH expression and continued cell division are not incompatible after oncogenic transformation.
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Alternate Webpage(s)	http://www.jneurosci.org/content/13/3/1280.full.pdf
Alternate Webpage(s)	http://www.jneurosci.org/content/jneuro/13/3/1280.full.pdf
PubMed reference number	7680068v1
Volume Number	13
Issue Number	3
Journal	The Journal of neuroscience : the official journal of the Society for Neuroscience
Language	English
Access Restriction	Open
Subject Keyword	5' Flanking Region Adrenal Glands Anabolism Base Pairing Cell division Cultured Cell Line Dopamine Flank (surface region) Intermediate Filaments Mixed Function Oxygenases Neoplasms Neurofilaments Neuroglia Norepinephrine Oncogenes SYP gene TH gene Transcription, Genetic Transcriptional Regulation Tyrosine 3-Monooxygenase
Content Type	Text
Resource Type	Article