NDLI: Développement et organisation de nanostructures : applications à l'exaltation des processus optiques pour la Biologie

Content Provider	Semantic Scholar
Author	Moal, Eric Le
Copyright Year	2007
Abstract	La fluorescence d'une molecule est sensible a son environnement electromagnetique. La presence d'une structure metallique modifie l'excitation et l'emission des fluorophores, par le jeu d'interferences et de couplages avec les plasmons de surface. Nous avons elabore et caracterise des films metalliques de differentes morphologies (films de nanoparticules, percoles, continus, plans, rugueux. . .). Leur influence sur la reponse optique des fluorophores a ete etudiee par la modelisation puis l'experience, en fonction de la distance fluorophore-metal et de l'orientation moleculaire. Une amplification d'un a deux ordres de grandeur du signal detecte est observee, ainsi qu'une photostabilisation des fluorophores et une modification des transferts d'energie intermoleculaires. Nous demontrons l'interet de cette technologie pour ameliorer la sensibilite dans les puces a ADN et pour l'imagerie des cellules et des tissus.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://pastel.archives-ouvertes.fr/tel-00164549/file/TheseChapitree.pdf
Alternate Webpage(s)	https://pastel.archives-ouvertes.fr/tel-00164549/document
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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