NDLI: Tissue-type plasminogen activator ( tPA ) is stored in Weibel-Palade bodies in human endothelial cells both in vitro and in vivo

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Tissue-type plasminogen activator ( tPA ) is stored in Weibel-Palade bodies in human endothelial cells both in vitro and in vivo

Content Provider	Semantic Scholar
Author	Huber, Denise Cramer, Elisabeth M. Kaufmann, Jocelyne E. Da, Paolo Massé, Jean-Marc Kruithof, Egbert K. O. Vischer, Ulrich M.
Copyright Year	2002
Abstract	Vascular endothelial cells are thought to be the main source of plasma tissue-type plasminogen activator (t-PA) and von Willebrand factor (VWF). Previous studies have suggested that both t-PA and VWF are acutely released in response to the same stimuli, both in cultured endothelial cells and in vivo. However, the subcellular storage compartment in endothelial cells has not been definitively established. We tested the hypothesis that t-PA is localized in Weibel-Palade (WP) bodies, the specialized endothelial storage granules for VWF. In cultured human umbilical vein endothelial cells (HUVECs), t-PA was expressed in a minority of cells and found in WP bodies by immunofluorescence. After up-regulation of t-PA synthesis either by vascular endothelial growth factor (VEGF) and retinoic acid or by sodium butyrate, there was a large increase in t-PA-positive cells. t-PA was exclusively located to WP bodies, an observation confirmed by immunoelectron microscopy. Incubation with histamine, forskolin, and epinephrine induced the rapid, coordinate release of both t-PA and VWF, consistent with a single storage compartment. In native [...] HUBER, Denise, et al. Tissue-type plasminogen activator (t-PA) is stored in Weibel-Palade bodies in human endothelial cells both in vitro and in vivo. Blood, 2002, vol. 99, no. 10, p.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.bloodjournal.org/content/bloodjournal/99/10/3637.full.pdf?sso-checked=true
Alternate Webpage(s)	https://archive-ouverte.unige.ch/files/downloads/0/0/0/3/5/7/7/9/unige_35779_attachment01.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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