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Detection of homozygous deletions of the cyclin-dependent kinase 4 inhibitor (p16) gene in acute lymphoblastic leukemia and association with adverse prognostic features.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Fizzotti, Marco Cimino, Giuseppe Pisegna, Simona Alimena, Giuliana Quartarone, Carmelo Mandelli, Franco Pelicci, Pier Giuseppe Coco, Francesco Lo |
| Copyright Year | 1995 |
| Abstract | A recently described putative tumor suppressor gene, the cyclin-dependent kinase 4 inhibitor (p16), has been shown to be altered by deletions and/or point mutations in various human cancers. To assess the incidence and clinico-biologic correlations of p16 homozygous deletion in hemopoietic tumors, we studied a panel of 244 DNA samples representative of distinct acute (99 cases) and chronic (57 cases) leukemia subtypes, myelodysplastic (22 cases) and myeloproliferative (15 cases) syndromes, and lymphomas (51 cases). A 361-bp probe complementary to the p16 exon 2 gene sequences was generated by polymerase chain reaction and used in Southern blot hybridization against these tumor DNAs. Homozygous deletions of p16 (p16-/-) were detected in 10 of 58 (17%) cases of acute lymphoblastic leukemia (ALL) of either B or T lineage and in no other tumors. Single-strand conformation polymorphism analysis of p16 exons 1 and 2 was also performed in 40 of the 58 ALL cases and in 16 lymphomas. In no cases were point mutations detected. The comparison of clinical features at presentation in p16-/- and in p16 germline ALL cases showed a greater leukemic cell mass (P = .001) and higher white blood cell counts (P = .01) in the former group. Two ALL cases in which diagnostic and relapse DNA samples were available showed p16-/- in both specimens. We conclude that homozygous p16 gene deletions characterize a subset of ALL with features of aggressive disease. |
| File Format | PDF HTM / HTML |
| DOI | 10.1182/blood.V85.10.2685.bloodjournal85102685 |
| PubMed reference number | 7742527 |
| Journal | Medline |
| Volume Number | 85 |
| Issue Number | 10 |
| Alternate Webpage(s) | http://www.bloodjournal.org/content/bloodjournal/85/10/2685.full.pdf?sso-checked=true |
| Alternate Webpage(s) | https://doi.org/10.1182/blood.V85.10.2685.bloodjournal85102685 |
| Journal | Blood |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
