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Biolistic inoculation of cassava (Manihot esculenta Crantz) with South African cassava mosaic virus
| Content Provider | Semantic Scholar |
|---|---|
| Author | Makwarela, Murunwa |
| Copyright Year | 2006 |
| Abstract | Cassava mosaic disease (CMD) is undoubtedly the most widespread disease in cassava (Manihot esculenta Crantz) in sub-Saharan Africa. While African cassava mosaic virus-Kenya (ACMV-KE), African cassava mosaic virus-Uganda (ACMV-UG), African cassava mosaic virus-India (ACMV-IC), East African cassava mosaic virus-Cameroon (EACMV-CM) and a recombinant East African cassava mosaic virus-India/Cameroon (EACMV-CM/IC) can be transmitted easily and repeatedly to Nicotiana benthamiana plants, difficulty in mechanical transmission and multiplication of EACMV-UG and South African cassava mosaic virus (SACMV) in N. benthamiana appears to place them biologically in a different group. In vitro grown 3-week-old cassava plants were biolistically inoculated with SACMV DNA A and B dimers and infectivity measured using a system based on visual assessment. We report for the first time successful infection, induced by SACMV DNA A and B dimer, of cassava cultivar TMS60444 using biolistic inoculation. Typical mosaic symptoms started to show at 14 days post-inoculation (dpi) in infected cassava plants, and SACMV replication was confirmed using PCR. Potential applications of biolistic infection of SACMV are also discussed. |
| Starting Page | 154 |
| Ending Page | 156 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| Volume Number | 5 |
| Alternate Webpage(s) | https://tspace.library.utoronto.ca/bitstream/1807/6654/1/jb06023.pdf |
| Alternate Webpage(s) | http://wiredspace.wits.ac.za/bitstream/handle/10539/1640/PhD%20Chapter%203%20Final.pdf?sequence=2 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
