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Identification and Functional Characterization of Long Non-coding RNA MIR22HG as a Tumor Suppressor for Hepatocellular Carcinoma
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, D. M. Zou, Xue-Jing Cao, C. Zhang, Ting Lei, Ling Qi, Xiao-long Liu, Li Wu, De-hua |
| Copyright Year | 2018 |
| Abstract | Long non-coding RNAs (lncRNAs) have recently been identified as critical regulators in tumor initiation and development. However, the function of lncRNAs in human hepatocellular carcinoma (HCC) remains largely unknown. Our study was designed to explore the biological function and clinical implication of lncRNA MIR22HG in HCC. Methods: We evaluated MIR22HG expression in 52-patient, 145-patient, TCGA, and GSE14520 HCC cohorts. The effects of MIR22HG on HCC were analyzed in terms of proliferation, invasion, and metastasis, both in vitro and in vivo. The mechanism of MIR22HG action was explored through bioinformatics, luciferase reporter, and RNA immunoprecipitation analyses. Results:MIR22HG expression was significantly down-regulated in 4 independent HCC cohorts compared to that in controls. Its low expression was associated with tumor progression and poor prognosis of patients with HCC. Forced expression of MIR22HG in HCC cells significantly suppressed proliferation, invasion, and metastasis in vitro and in vivo. Mechanistically, MIR22HG derived miR-22-3p to target high mobility group box 1 (HMGB1), thereby inactivating HMGB1 downstream pathways. Additionally, MIR22HG directly interacted with HuR and regulated its subcellular localization. MIR22HG competitively bound to human antigen R (HuR), resulting in weakened expression of HuR-stabilized oncogenes, such as β-catenin. Furthermore, miR-22-3p suppression, HuR or HMGB1 overexpression rescued the inhibitory effects caused by MIR22HG overexpression. Conclusion: Our findings revealed that MIR22HG plays a key role in tumor progression by suppressing the proliferation, invasion, and metastasis of tumor cells, suggesting its potential role as a tumor suppressor and prognostic biomarker in HCC. |
| Starting Page | 3751 |
| Ending Page | 3765 |
| Page Count | 15 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.thno.org/v08/p3751/thnov08p3751s1.pdf |
| Alternate Webpage(s) | http://www.thno.org/v08p3751.pdf |
| PubMed reference number | 6071531 |
| Alternate Webpage(s) | https://doi.org/10.7150/thno.22493 |
| DOI | 10.7150/thno.22493 |
| Journal | Theranostics |
| Volume Number | 8 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Biological Markers Chromosome 3 Short Arm HMGB1 Protein HMGB1 gene Liver carcinoma Luciferases MIR22 wt Allele MIR22HG gene Neoplasms Oncogenes Patients The Cancer Genome Atlas Tumor Initiation Tumor Progression long noncoding RNA Fas-AS1, human non-T, non-B childhood acute lymphoblastic leukemia non-T, non-B, cALLa negative childhood acute lymphoblastic leukemia pediatric intracranial germ cell brain tumor tumor suppressor activity |
| Content Type | Text |
| Resource Type | Article |
