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Myocardial creatine kinase expression after left ventricular assist device support.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Park, Soon Joo Zhang, Jianyi Jay Ormaza, Sofia Liang, Peihua |
| Copyright Year | 2002 |
| Abstract | OBJECTIVES We examined whether unloading of the left ventricle with a ventricular assist device (LVAD) can result in normalization of the creatine kinase (CK) abnormalities in the failing human heart. BACKGROUND Left ventricular failure is associated with a decrease of myocardial total CK activity and a fetal shift in CK isoform expression that results in an increase in the cytosolic brain type homodimeric-creatine kinase (CK-B) subunit and decreases of the cytosolic muscle-creatine kinase (CK-M) and CK-mitochondrial (CK-Mt) isoforms. The mechanisms of this abnormality are not known. METHODS Total CK activity and CK protein isoform expression (Western blotting) were examined in 11 patients with end-stage cardiomyopathy. In 7 patients, myocardial tissue was also obtained after 4.1 +/- 1.1 months of left ventricular assist device (LVAD) support. RESULTS Left ventricular unloading produced by LVAD implantation resulted in a 270% +/- 114% increase in total CK activity (p < 0.01) that was associated with a 69% +/- 18% increase in CK-M protein expression (p < 0.01) and a 121% +/- 69% increase in CK-Mt protein expression (p < 0.01), but no significant change in CK-B expression. CONCLUSIONS Systolic and diastolic unloading provided by the LVAD resulted in increases of total CK activity as well as CK-Mt and CK-M protein expression. The failure of CK-B expression to decrease suggests that abnormalities other than increased loading are responsible for the increase in CK-B expression in the failing heart. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.onlinejacc.org/content/accj/39/11/1773.full.pdf |
| PubMed reference number | 12039490v1 |
| Volume Number | 39 |
| Issue Number | 11 |
| Journal | Journal of the American College of Cardiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cardiomyopathies Congenital Abnormality Creatine Kinase Diastole Heart Ventricle Left ventricular structure Liver Failure, Acute Muscle Myocardial Infarction Myocardium Patients Protein Isoforms Sixty Nine Ventricular failure Western Blotting left ventricular assist device protein expression |
| Content Type | Text |
| Resource Type | Article |
