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Effects of temperature acclimation on Pacific bluefin tuna (Thunnus orientalis) cardiac transcriptome.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jayasundara, Nishad Gardner, Luke David Block, Barbara A. |
| Copyright Year | 2013 |
| Abstract | Little is known about the mechanisms underpinning thermal plasticity of vertebrate hearts. Bluefin tuna hearts offer a unique model to investigate processes underlying thermal acclimation. Their hearts, while supporting an endothermic physiology, operate at ambient temperature, and are presented with a thermal challenge when migrating to different thermal regimes. Here, we examined the molecular responses in atrial and ventricular tissues of Pacific bluefin tuna acclimated to 14°C, 20°C, and 25°C. Quantitative PCR studies showed an increase in sarcoplasmic reticulum Ca(2+) ATPase gene expression with cold acclimation and an induction of Na(+)/Ca(2+)-exchanger gene at both cold and warm temperatures. These data provide evidence for thermal plasticity of excitation-contraction coupling gene expression in bluefin tunas and indicate an increased capacity for internal Ca(2+) storage in cardiac myocytes at 14°C. Transcriptomic analysis showed profound changes in cardiac tissues with acclimation. A principal component analysis revealed that temperature effect was greatest on gene expression in warm-acclimated atrium. Overall data showed an increase in cardiac energy metabolism at 14°C, potentially compensating for cold temperature to optimize bluefin tuna performance in colder oceans. In contrast, metabolic enzyme activity and gene expression data suggest a decrease in ATP production at 25°C. Expression of genes involved in protein turnover and molecular chaperones was also decreased at 25°C. Expression of genes involved in oxidative stress response and programmed cell death suggest an increase in oxidative damage and apoptosis at 25°C, particularly in the atrium. These findings provide insights into molecular processes that may characterize cardiac phenotypes at upper thermal limits of teleosts. |
| Starting Page | 228 |
| Ending Page | 236 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ajpregu.physiology.org/content/ajpregu/305/9/R1010.full.pdf |
| Alternate Webpage(s) | http://www.tunaresearch.org/reprints/Jayasundara_AJPRICP_2013.pdf |
| PubMed reference number | 24005253v1 |
| Alternate Webpage(s) | https://doi.org/10.1152/ajpregu.00254.2013 |
| DOI | 10.1152/ajpregu.00254.2013 |
| Journal | American journal of physiology. Regulatory, integrative and comparative physiology |
| Volume Number | 305 |
| Issue Number | 9 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Acclimatization Adenosine Triphosphate Antiapoptotic Agent Apoptosis Artificial cardiac pacemaker Body tissue Calcium Cell Death Cell Survival Cellular Stress Response Cessation of life Cold Temperature Energy Metabolism Excitation Gene Expression Heart Atrium Heat Stress Disorders Mammals Metabolic Process, Cellular Molecular Chaperones Muscle Cells Myocytes, Cardiac Oxidative Stress PO-2 Phenotype Principal Component Protein Degradation, Metabolic Reproduction Reproductive Fitness SCARA3 gene Sarcoplasmic Reticulum Thunnus orientalis Thunnus thynnus Transcriptome Tuna (fish) biological adaptation to stress cellular homeostasis enzyme activity heart function physiological aspects |
| Content Type | Text |
| Resource Type | Article |
