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Micropropagation of seabuckthorn (Hippophae rhamnoides ssp. turkestanica).
| Content Provider | Semantic Scholar |
|---|---|
| Author | Singh, Virendra Gupta, Rajesh |
| Copyright Year | 2014 |
| Abstract | Studies on the development of protocol was conducted on the Lahaul form of H. rhamnoides ssp. turkestanica), the most promising form of the Himalayan seabuckthorn. With active bud explants of seabuckthorn, more than 95% contamination free cultures were established through surface sterilization scheme of 0.1% detergent (2 Hours), Tetracycline (2 Hours), 70% EtOH (4 Min) and 0.1 % HgCl2 (6 Min), whereas with dormant buds 0.1% detergent (5 Hours), Tetracycline (over night), 70% EtOH (15 Min) and 0.1 % HgCl 2 (18 Min). There was increase in overall explant survival from MS through ½MS to WPM, and decrease in explant vitrification from ½MS through MS to WPM. Vitrifi- cation level decreased from MS to WPM. Comparison of media-hormone interaction revealed that e xplant survival was highest, on WPM medium (80.6%), closely followed by ½MS (80%) and lowest on MS medium. Overall on MS medi- um, % multiple shoot, shoot/explant and callusing decreased along successive passages. On ½ MS maximum shoots/explant of a maximum of 1.4 shoots/explant was observed in combination BAP 0.2: IBA 0.01 in 60% of the cu l- tures, followed by maximum of 1.1 shoots/explant in BAP 0.2: IBA 0.01 in 40% of the cultures during first stage of cu l- turing passage (PI). On WPM medium, in 60% of the cultures, shoots/explant with a maximum of 14.6 shoot/explant was observed in BAP 1.0: IAA 0.5 ppm, which was followed by 66.7% cultures having maximum of 5 shoot/explant in BAP 0.3: NAA 0.2 ppm combination during PI. Overall there was increase in % multiple shooting, shoots/explant and callu s- ing along successive passages on WPM Medium. Comparison of multiple shoot development on different culture media revealed that there was increase in % multiple shoot development and shoot/explant across different culture media from MS through ½MS to WPM along successive passages. Maximum shoot survival of 83.3% was observed with IBA 1.0 ppm. Highest root induction of 66.7% was observed with IBA 1.5 ppm. Among different culture media tested with var i- ous growth hormone combinations, WPM medium with 3% sucrose, was found to be suitable for the induction of mult i- ple shoots, with hormone combination of BAP 1.0: IAA0.5 ppm and WPM with 2% sucrose and 1.5 ppm IBA was found to be suitable for the induction of rooting in seabuckthorn shoots. Further work is in progress to improve the multiple shoot frequency as well as improvement of rooting induction. |
| Starting Page | 131 |
| Ending Page | 139 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Volume Number | 4 |
| Alternate Webpage(s) | http://openaccessscience.com/pdf-files/vol4_2_jun2014/IJMAP_4_2_8_Hippophae_rhamnoides.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
